南极磷虾抗氧化多肽制备的研究

Preparation of Antarctic krill peptides with anti-oxidant activity

以清除超氧阴离子自由基、DPPH自由基和羟基自由基能力为指标，筛选制备南极磷虾杭氧化多肽的水解条件，得到高效的抗氧化多肽制品。结果表明：胰蛋白酶、中性蛋白酶复合，酶解南极磷虾6h后得多肽的抗氧化能力最强，超氧阴离子自由基的清除率达到11．5％，DPPH自由基的清除率达到72．7％，羟基自由基的清除率达到84．8％。经超滤分离，分子量范围在5-10ku的多肽表现出较强的清除三种自由基的能力。对其进行tricine—SDS—PAGE电泳，结果显示电泳图谱上出现了两个条带。

In the study, the free radical scavenging ability against superoxide, DPPH and hydroxyl radical was investigated to screen optimum hydrolysis conditions for preparing Antarctic krill peptides with high anti-oxidant activity.Results indicated that peptides prepared by trypsin and neutral protease for 6h,showed the highest anti- oxidant activity,with free radical scavenging effect on superoxide, DPPH and hydroxyl radical by 11.5% ,72.7% and 84.8%, respectively.Through ultra-filtration, peptides （5-10ku） exhibited stronger scavenging activity against free radicals,which further revealed with two bands in the tricine-SDS-PAGE electrophoretogram.