茶皂素还原脱色法初探

Research of blanching sasanquasaponin by reduction agents

实验通过还原脱色方法对茶皂素溶液进行脱色研究,取得了较好的效果。实验确定了四个参数:两种脱色剂的使用量(KBH4和NaHSO3)、pH以及脱色温度。通过响应面进行优化,最终优化的结果为:两种脱色剂KBH4、NaHSO3的添加量分别为1.2%(w/w)和2.2%(w/w),pH为4.8,脱色温度为51.0℃,该优化条件下ΔE值为31.324±0.378,得率为95.8%。使用LC-MS/MS对脱色后的样品进行测定,结果表明还原脱色法对茶皂素结构没有影响。

This study was to decolorize sasanquasaponin （SQS）solution effectively by reduction method. The response surface methodology （RSM）was used to optimize conditions for blanching SQS solution.The following parameters：dosage of blanching agents（ KBH4 and NaHSQ ）, pH of the solution and reaction temperature were the variables.The optimal conditions determined by response surface methodology were：the dosage of KBH4 was 1.2% （w/w） ,the dosage of NaHSO3 was 2.2% （w/w）, pH of 4.8 and reaction temperature of 5L0℃.The △E value under the optimal condition was 31.324 ± 0.378 and the yield of sasanquasaponin was 95.8%.Further detection of LC-MS/MS showed that bleaching agents did not degrade the structure of sasanquasaponin.