花椒麻味素剂量对SD雌性大鼠肠道健康的影响

Effects of sanshool on the intestinal health effects of female Sprague-Dawley rats

目的:研究不同花椒麻味素灌喂剂量对大鼠肠道健康的影响。方法:将40只SD雌性大鼠按体重随机将大鼠分为空白组(LOD),高脂组即高脂空白组(HF-LOD)、高脂并灌胃4mg/kg/d(HF-CD)、高脂并灌胃8mg/kg/d(HF-MD)、高脂并灌胃12mg/kg/d(HF-HD)花椒麻味素的大豆油溶液等共5组各8只。饲养期间自由采食基础饲料和饮水,喂养4周后解剖,测定盲肠质量、盲肠内容物、盲肠组织重量、盲肠内容物游离氨以及盲肠中短链脂肪酸和微生物。结果:高脂空白组与空白组相比,采食量、饲料效率和盲肠面积显著增加,双歧杆菌显著降低;高脂空白组与剂量组相比,各剂量组的体重增加量、饲料效率、乳酸菌、厌氧菌和双歧杆菌显著增加,盲肠面积显著降低,中剂量组的丙酸、异丁酸、总的SCFAs显著增加,肠球菌显著降低,中、高剂量组的大肠杆菌显著降低。结论:高脂饲料会延长饲料在消化道的时间,抑制肠道有益菌的生长,花椒麻味素能够改善由高脂饲料引起的各种肠道不良反应,促进肠道发酵和有益菌的生长。

Objective：To study the effect of different dose of sanshool on rats＇ intestinal health.Methods.40 female SD rats were randomly divided into 5 groups of 8 according to the weight, which included control group rats （ LOD）, high-fat group namely blank group（ HF-LOD）, high-fat fed 4mg/kg/d （ HF-CD）, high-fat fed 8mg/kg/d （HF-MD） ,high-fat fed ]2mg/kg/d（HF-HD） Sanshool Solubled in Soybean oil.During the feeding ,feed and water intake freely, after 4 weeks of feeding, cecum quality, cecal contents, cecal tissue weight, cecal contents, free ammonia,short chain fatty acids and microorganism in the cecum were determined.Results ：Contrasted with blank control group,feed intake,feed efficiency and cecal area in high-fat group increased significantly,bifidobacterium significantly reduced.Compared with the control group, each dose of the weight gain, feed efficiency, lactic acid bacteria,anaerobic bacteria and bifidobacteria of the high-fat group increased significantly, the cecal area significantly reduced, propionic acid, isobutyric acid,total SCFAs in the middle dose group increased significantly, enterococci significantly reduced, E. coli in the middle and high dose group significantly reduced. Conclusion： High-fat diet will extend the digestion and inhibit the growth of intestinal bacteria,Sanshool can improve a variety of intestinal bad reactions caused by the high fat diet and promote intestinal fermentation and the growth of beneficial bacteria.