黑曲霉孢子粉真空冷冻干燥保护剂配方优化

Optimization of Cryoprotectant Formulation for Vacuum Lyophilization of Spore Power of Aspergillus niger

采用单因素试验和混合均匀试验设计,对黑曲霉孢子粉真空冷冻干燥保护剂配方进行筛选和优化。通过Design-Expert软件对实验数据进行回归分析,得到保护剂最佳配方为脱脂奶粉6.43 g/100 mL、蔗糖13.03 g/100 mL、甘油6.88 g/100 mL,孢子存活率预测值为88.82%,实际值为87.03%。

A cryoprotectant composed of skim milk powder, sucrose and glycerol for vacuum lyophilized spore power of Aspergillus niger was developed and its formulation was optimized by one-factor-at-a-time and uniform design. By regression analysis of the experimental data, the optimal cryoprotectant formulation （per 100 mL of cryoprotectant） found to consisted of 6.43 g of skim milk powder, 13.03 g of sucrose, and 6.88 g of glycerol. The predicted survival rate of spore with the addition of the optimized cryoprotectant after freeze-drying was 88.82%, which was close to the actual value of 87.03%.