不同病变胃粘膜DNA含量与癌基因表达的检测

Detection of the DNA content of stomach mucosa with pathologic changes and oncogene expresion

用图像分析技术检测143例胃的慢性炎症、肠上皮化生、异型增生、癌各组细胞核DNA含量及倍体分布情况，并同时进行nm23、PCNA、 C-erbB-2抗体染色。结果显示nm23的阳性率在４种病变中无明显差异， DNA含量、PCNA、C－erbB-2则呈递增表达（P＜0.01）；nm23阴性表达、 PCNA与C-erbB-2阳性表达的细胞核DNA含量与５倍体(5c)及非整倍体(AN)检出率显著高于nm23阳性、PCNA、C-erbB－2阴性表达者（P＜0.05）。提示胃良恶性病变细胞核中DNA含量及倍体分布形式不同，nm23失表达、 PCNA与C－erbB-2过度表达与5c和AN细胞形成有关，形态定量分析及癌基因检测可作为胃粘膜上皮病变诊断和分类的综合性指标。

We use 143 cases of stomach pathological change whose gene expression show negative,positive(including 20 cases of gastritis,16 cases of intestinal me taplasia,34 cases of dysplasia,73 cases of carcinom) DNA image analysis can be u sed to test the DNA content of nuclear.the positive percentge of nm23 decreases progressirely among four pathological changes and the DNA content,PCNA,C-erbB -2 incerase by regular degrees.The DNA content of nuclear of nm23 nositive,PCN A,C-erbB-2 positive and percinlages of aneuploidy is evidently higher than tha t of nm23 positive,PCNA,C-erbB-2 nositive( P ＜0.05).The DNA content of nu clear in stomach benign and malignant pathological changes is different.Image a nalysis technique cancer genes can be used as a comprehensive index to diagnose and classify pathological changes of gastric mucosa and can be helptul to conduc t clinical treatment and estimate the malignant potential of tumor and prevent o ther pathologucal changes.