HPLC同时测定保和颗粒中3种化学成分的含量

Simultaneous Determination of Three Kinds Chemical Contents in Baohe Granules by HPLC

目的：建立同时测定保和颗粒中芦丁、橙皮苷和连翘苷含量的方法。方法：应用高效液相色谱法测定，色谱柱为AgilentZorbax SB-C18色谱柱（4．6mm×250mm，5μm）；流动相为乙腈-0．05％磷酸溶液，梯度洗脱：流速为1．0mL·min^-1，柱温30℃；检测波长277nm。结果：芦丁、橙皮苷和连翘苷的线性范围分别为1．741-130．6μg·mL^-1（r=0．9992），1．687～126．5μg·mL^-1（r=0．9998），0．6304～47．28μg·mL^-1（r=1．0000）；平均加样回收率分别为100．92％（RSD=1．05％），100．87％（RSD=1．45％），99．86％（RSD=1．73％）。结论：该方法同时测定保和颗粒中的芦丁、橙皮苷、和连翘苷的含量，简便易行、准确、重复性好，可用于保和颗粒的质量控制。

Objective ：To establish a high performance liquid chromatographic method for simultaneous determinationthe contents of Rutin, Hesperidin and Phillyrin in Baohe Granules. Methods ： HPLC was applied with the chromatographic conditions as follows：the chromatographic column was Agilent Zorbax SB -C 18 （4.6 mm × 250 mm,5 μm） at 30 ℃ ; acetonitrile -0.05 % H3 PO4 as the mobile phrase, gradient elution;flow rate was 1.0 mL . min^- 1, the detection wavelength was 277nm. Results ： Linearity relationships were 1. 741 - 130.6 μg . mL^- 1 （ r = 0. 9992） , 1. 687 - 126.5 μg . mL ^- 1 （ r =0. 9998） ,0. 6304 - 47.28 μg . mL^-1 （ r = 1. 0000）, respectively; the average recoveries were 100.92% （ RSD = 1.05% ）, 100.87% （ RSD = 1.45% ） ,99.86% （ RSD = 1.73% ）. Conclusion：The results showed that the method was simple, accurate and repeatable and it was suitable for simultaneous determination of the contents of Rutin, Hesperidin and Phillyrin in Baohe Granules.