RP-HPLC法同时测定注射用丹心宁中4个成份的含量

Determination of Four Compounds from Zhusheyong Danxinning by RP-HPLC

目的：建立同时测定注射用丹心宁中丹参素、原儿茶醛、丹酚酸B、红花黄色素含量的反相高效液相色谱法。方法：以Agilent Zorbax Extend C18柱（250mm×4．6mm，5μm）为固定相，乙腈-0．03％磷酸为流动相．在0～65min，乙腈量从2％上升到33％，检测波长为280nm和403nm，流速0．8mL·min^-1。结果：4种成份分离良好，丹参素、原儿茶醛、丹酚酸B、红花黄色素进样量分别在范围0．313—3．756μg，0．053-0．6362μg．0．567—6．809μg和0．0443—0．5321μg线性关系良好，加样回收率分别为丹参素102．1％，RSD=1．1％；原儿茶醛97．3％，RSD=1．2％；丹酚酸B99．3％，RSD=1．2％；羟基红花黄色素A100．6％，RSD=1．5％。结论：本法简单、快捷、适合于测定注射用丹心宁中丹参素、原儿茶醛、丹酚酸B、羟基红花黄色素A的含量。

Objective ：To develop a RP -HPLC method for the first time to simultaneously determine four major compounds in Zhusheyong Danxinning, namely danshensu, protocatechuic aldehyde, salvianolic acid B and hydroxysafflor yellow A with UV detection at 280 nm and 403 nm. Methods ： The column was Agilent Zorbax Extend C 18 （250 mm × 4.6 mm,5 μm）. The separation was carried out with a gradient program. The mobile phase was acetonitrile- 0.03% phosphoric acid at a flow rate of 0.8 mL . min^-1. Results ：The standard curve was rectilinear in the range of 0. 313 -3. 756 μg （ r = 1. 00130） for danshensu ,0. 053 - 0. 6362 μg （ r = 1. （3000） for protocatechuic aldehyde,0. 567 - 6. 809 μg （ r = 1. 0000） for salvianolic acid B, and 0. 0443 - 0. 5321 μg （ r = 1. 0000） for hydroxysafflor yellow A. The recoveries of the markers listed above were 102.1% ,97.3 % ,99.3% , and 100.6% , respectively. Conclusion：The method is simple, rapid and suitable for the determination of four compounds in Zhusheyong Danxinning.