抗纤颗粒防治肝纤维化大鼠作用机理探讨

STUDY ON MECHANISM OF KANGXIANKELI PREVENTING AND CURING HEPATIC FIBROSIS

目的 :动态观察抗纤颗粒对大鼠肝纤维化模型细胞因子与肝组织胶原蛋白的影响 ,进而探讨抗纤颗粒防治肝纤维化的作用机制。方法 :将SD大鼠随机分为 10组 ,即正常对照 4W (W :Week周 )组、8W组、模型对照4W组、8W组、纤低预防 4W组、8W组、纤高预防 4W组、8W组、纤低治疗 8W组、纤高治疗 8W组。以二甲基亚硝胺(Dimethylnitrosamine ,DMN)腹腔注射建立肝纤维化模型 ,除正常对照组与模型对照组外 ,其余各组给予抗纤颗粒灌胃处理。各 4W组于实验第 30天、8W组于实验第 6 0天处死动物 ,取血作肝功能 ;TNF -α、IL - 1α、γ -IFN ;取肝组织作胶原蛋白测定。结果 :①抗纤颗粒能显著提高各期模型大鼠血清中γ -IFN含量和显著降低各期模型大鼠血清中TNF -α、IL - 1α活性及肝胶原蛋白含量 ;②TNF -α、IL - 1α与肝胶原蛋白呈显著正相关关系 (P <0 0 1,r>0 5 ) ,γ-IFN与肝胶原蛋白呈显著负相关关系 (P <0 0 1,r<- 0 5 )。结论 :抗纤颗粒预防和治疗肝纤维化的机理 。

Objective:To study the mechanism of Kangxiankeli in preventing and curing hepatic fibrosis.Methods:SD rats were randomly divided into 10 groups, namely,normal control 4W (weeks) group and 8W group, model control 4W group and 8W group, low preventive dosage 4W group and 8W group, high preventive dosage 4W group and 8W group, low curative dosage 8W group and high curative dosage 8W group. Each group comprises 10 rats.Rat hepatic fibrosis model was produced by Dimethylnitrosamine(DMN) injection into the belly cavity.All of the subjects except those in control groups were perfused with Kangxiankeli into stomach.Animals were killed 4 weeks or 8 weeks after experiment. Liver function was tested , TNF-α, IL-1α,γ-IFN in serum were detected and collagen in liver tissue was determined.Results:Kangxiankeli could remarkbaly elevate serum γ-IFN level while lower TNF-α, IL-γ and cut down the content of collagenous protein in liver tissue . TNF-α and IL-1γ were positive correlated(P<0.01) ,while γ-IFN was negatively correlated (P<0.01) ,with collagenous protein.Conclusion:The Kangxiankeli's effect on hepatic fibrosis is suggested to be through regulating the cytokine net.