地龙组分促进痔术后创面愈合的临床和实验研究

Study on Lumbricus in Promoting Ligation Hemorrhoidectomy Postoperative Wound Healing

目的 :探讨地龙对痔术后创面愈合的影响。方法 :将人工养殖的鲜地龙制成的溶液 (创愈新 )喷洒于混合痔手术后患者 (治疗组 )创面 ,并与使用紫草药条 (对照组 )作比较 ,观察愈合情况 ;同时观察了创愈新对创伤动物创面愈合的影响 (实验组 )及组织学变化 ,并与 0 1%雷夫奴尔治疗组 (对照组 )作比较。结果 :临床研究结果显示 :创面愈合时间治疗组平均为 ( 16 5± 1 8)天 ,较对照组〔( 2 1 2± 2 8)天〕明显缩短 (P<0 0 1) ;用药后 3天内表皮生长治疗组较对照组明显加快 (P <0 0 1) ;无创面感染及肉芽过度增生。实验研究结果显示 :创愈新有抑制炎症作用 ,对金黄色葡萄球菌、大肠杆菌、绿脓杆菌有抑制作用。愈合时间实验组较对照组提前 4天。第 4、7天处死的兔毛细血管数、血管腔内皮细胞数及间质成纤维细胞数实验组较对照组明显增多。上皮细胞分裂象、第 4天间质细胞分裂象及第 7天细胞核分裂指数实验组均高于对照组。自第 3天开始创面愈合及肉芽填充速度实验组明显快于对照组。结论 :本制剂价廉 ,易保存 ,可用于促进创面愈合 ,无毒、副作用。

To investigate the effect of Lumbricus in promoting wound healing of ligation hemorrhoidectomy. Methods: Spray the artificial grown fresh Lumbricus solution on the wound surface of the mixed hemorrhoid patients (treated group) after hemorrhoidectomy and also on wound of the experimental animals, and Lithospermum erythrorhizon medicated gauze was taken as control to observe the effect of treatment on wound healing and histologic change. Results: The mean wound healing time of the treated group was 16.5±1.8 days, as compared to the control group (21.2±2.8 days), it was obviously shortened (P<0.01), 3 days after medication, the growth of epidermis of the treated group was obviously more rapid than that of the control group, no wound infection and granulation hyperplasia were found. The experimental study showed that Lumbricus preparation could inhibit inflammation, bacteria such as Staphylococcus aureus, coli and proteus bacillus was inhibited. In comparson with the control group, the experimental group shortened the healing period for 4 days. On the 4th and 7th day, sacrificed rabbits′ cappillary count, vessel lumen endothelial cell count, and mesenchymal fibroblast count of the experimental group grower obviously than those of the control group, on the 4th day, the mesenchymal cell mitosis of the experimental group was higher than that of the control group, while on the 7th day, the cell nucleus mitotic index in the experimental group also was higher than that in the control group, from the 3rd day on, wound healing and granulation filling speed of the experimental group obviously more rapid than that of the control group. Conclusion: The Lumbricus preparation is cheap in price, easy to preserve, can be used in promoting wound healing, without any toxic and side-effects.