肾康注射液对肾小管上皮细胞转化生长因子-βmRNA基因表达的影响

Effect of Shenkang Injection on Transforming Growth Factor-β Messenger Ribonucleic Acid of LLC-PK_1 Renal Tubular Epithelial Cells

目的 :探讨肾康注射液对体外培养的肾小管上皮细胞LLC PK1转化生长因子 βmRNA(TGF βmRNA)基因表达的影响。 方法 :用逆转录聚合酶链反应 (RT PCR)、Northern印迹杂交 (Northernblot)方法 ,以单味大黄注射液为实验对照组 ,检测肾康注射液对肾小管上皮细胞TGF βmRNA基因表达的影响。结果 :肾康注射液可以明显下调肾小管上皮细胞TGF βmRNA基因的表达 ,并呈剂量依赖关系 ,肾康注射液作用明显优于同等含量的单味大黄注射液。结论 :肾康注射液下调肾小管上皮细胞TGF βmRNA基因表达 ,是其防治慢性肾功能衰竭 (CRF)的机理之一。

To study the effect of Shenkang injection (SKI) on the transforming growth factor (TGF) -βmRNA of LLC-PK 1 renal tubular epithelial cells. Methods: Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern Blot to observe the effect of SKI on the TGF-βmRNA of LLC-PK 1 renal tubular epithelial cells, with control group treated with the injection of Rheum officinale extraction. Results: SKI showed the inhibition of TGF-βmRNA expression of renal tubular epithelial cells, which is dose-dependent and was more obvious than that of the Rheum extractive injection with equal dose of drug. Conclusion: The mechanism of delaying chronic renal failure with SKI is achieved by down regulating TGF-βmRNA expression of renal tubular cells