应用PAGE技术分析武汉市婴幼儿轮状病毒分子流行病学特征

Analysis of Molecular Epidemiology of Rotavirus in Infants in Wuhan by PAGE

对１９９６年４月～１９９７年３月期间就诊于武汉市儿童医院的１６８３例门诊腹泻患儿粪便标本进行了轮状病毒ＲＮＡ的聚丙烯酰胺凝胶电泳分析。在５１５份标本中检出了轮状病毒ＲＮＡ，阳性率为３０．６０％。所检出的轮状病毒２个组中，以第二亚组多见，占９８．２５％，２个亚组又可进一步分为五种电泳型，即Ｓ、Ｌ１、Ｌ２、Ｌ３、Ｌ４型，５种电泳型中以Ｌ１型为主，占轮状病毒ＲＮＡ阳性病例的５６．１２％。对患儿临床表现、年龄与阳性率进行比较发现，呕吐、咳嗽、粪检时镜下脂肪球的发生均与轮状病毒感染密切相关（Ｐ＜０．００５），且发病以２岁内为易感年龄。

The polyacrylamide gel electrophoresis (PAGE) was performed to detect the rotavirus (RV) RNA in 1683 fecal samples of the diarrhea children who went to Wuhan Children Hospital from April 1996 to March 1997. RV RNA was detectable in 515 samples with the positive rate being 30. 60 %. RV in the 2 sub- groups were found and in the RV accounted for 98. 25 % of the all positive samples. In the 5 RV RNA elec- trophoresis patterns (S. L1, L2, L3, L4), L1 accounted for 56. 12 % of the all positive samples. The inci- dences of vomit, cough and fat droplets under microscopic screening were closely associated with the RV infec- tion (P<0. 005). The sensitive age for children to be infected with RV was under 2 years.