鼻咽癌放疗前及放疗中细胞凋亡的变化和意义

The Relationship between Spontaneous Apoptosis and Radiation-induced Apoptosis of Nasopharyngeal Carcinoma

目的 探讨鼻咽癌放疗前及放疗中细胞凋亡的变化及其意义。方法 采用TdT酶介导的生物素化dutp缺口末端标记技术 (TUNEL) ,检测 35例鼻咽癌患者放疗前及放疗中鼻咽部刷落癌细胞的细胞凋亡率 (apoptosisrate ,AR)。结果 鼻咽癌放疗前的AR为 7 2 %± 2 4 % ;放疗量分别为 10GY、30GY和 60GY时的AR分别为 64 3%± 2 1 4 %、57 2 %± 17 3%、16 2 %± 7 4 % ,明显高于放疗前 (P <0 0 1) ;放疗前的自发性细胞凋亡与放射治疗诱发的细胞凋亡呈正相关。结论 检测鼻咽癌患者刷落癌细胞的细胞凋亡率有助于判断放射敏感性。

To study the relationship between spontaneous apoptosis and radiation-induced apoptosis of nasopharyngeal carcinoma(NPC).Methods Exfoliated cells obtained from 30 cases of NPC before and during radiotherapy 10、30and 60GY were used in this study.Using terminal oxynucleotidyl transferase mediated dUTP biotin nick end labeling(TUNEL),the spontaneous and radiation-iuduced apoptoic rate(AR)were examined.Results The AR of exfoliated cells of NPC before and during radiotherapy 10,30 and 60GY was 7 2%±2 4%,64 3%±21 4%,57 2%±17 3% and 16 2%±7 4% respectively,the radiation-induced apoptotic rate was significantly higher than that of spontaneous apoptotic rate( P <0 01).In NPC,spontaneous apoptosis was positively correlated with the radiation-induced apoptosis. Conclusion Detecting the AR of exfoliated cells of NPC before and during radiotherapy may be helpful to predict the sensitivity to radiotherapy in NPC patients.