瘤内注射吉西他滨治疗大鼠脑胶质瘤的效果观察

Gecitabine intratumoral administration suppresses malignant glioma in rat

目的 观察吉西他滨瘤内给药治疗大鼠脑胶质瘤的效果,探讨治疗脑胶质瘤的有效化疗途径及药物.方法 50只SD大鼠随机分为对照组（5只）,肿瘤组和治疗（1 mg/ml）组和治疗（4mg/ml）组,每组15只.除对照组外,所有大鼠均在纹状体部埋植导管,并接种9L胶质瘤细胞株以建立在体脑胶质瘤模型.治疗组于植入瘤细胞第9天,通过导管将吉西他滨（10μl,1 mg/ml和4 mg/ ml）分别注入瘤内.于给药第9天,每组随机取脑抽检（5只）,观察瘤形态及大小;余者（每组10只）观察其饮食、行为、体质量及存活期,评价吉西他滨对大鼠脑胶质瘤的治疗效果.结果 于瘤内注入吉西他滨第9天取脑,发现各组肿瘤大小分别为：肿瘤组（44.69±7.52） mm3、治疗（1 mg/ml）组（22.44±5.37） mm3和治疗（4 mg/ml）组（18.72±7.13）mm3,肿瘤组与两治疗组间差异有统计学意义（P＜0.05）,但两治疗组之间差异无统计学意义（P＞0.05）.治疗组大鼠体质量下降较肿瘤组为轻.肿瘤组平均存活期为23.8d.4 mg/ml组1只存活期比肿瘤组短,剩余4只及全部1 mg/ml组（10只）均长于肿瘤组.结论 吉西他滨可抑制脑胶质瘤生长,缓解大鼠体质量下降,延长模型动物存活期,对大鼠脑胶质瘤具有抑制作用.

Objective To determine the possible efficacy of gecitabine against malignant glioma in the model rat to give some clues for clinical treatment.Methods Malignant glioma models were set up by implanting 9 L cells in striatum in rats.50 model rats were divided randomly into control group （5 rats）,tumor group,1 mg/ml group and 4 mg/ml group with 15 rats in each.Gecitabine （10 μl,1 mg/ml or 4 mg/ml） was delivered into the tumor by catheter at 9th day after the implanting of 9 L cells.5 rats from each group were collected randomly to detect the size of tumor in brain at 9th day after the administration of gecitabine.For the rest rats,the status abous eating,drinking,weight and life span （after 9 L cells were implanted） were recorded respectively.Results The size of tumors under gecitabine administration was smaller than that of the tumor group [tumor group （44.69±7.52） mm3,1 mg/ml group （22.44±5.37） mm3,4 mg/ml group （18.72±7.13） mm3],there were satestically significances between tumor group and gecitabine groups （P ＜ 0.05）,but there was not between the two gecitatine groups （P ＞ 0.05）.The losses of weight in gecitabine groups were minored compared with tumor group.The mean survival of the rats in tumor group was 23.8 d.Except for 1 rat in 4 mg/ml gecitabine group,the survivals of the other rats in 4 mg/ml group and all rats in 1 mg/ml group were longer than that in tumor group.Conclusion Gecitabine intratumor administration can inhibite the growth of malignant glioma,attenuate the decrease of weight and extend the life span of the model rats effectively.