磷酸二酯酶-4抑制剂对烟雾诱导的COPD大鼠肺组织中MUC5AC表达的影响及其作用机制的研究

The effect and the mechanism of phosphodiesterase 4 on MUC5AC expression in cigarette smoking-induced COPD rat lung tissue

目的:探讨磷酸二酯酶-4抑制剂罗氟司特对吸烟鼠肺组织MUC5AC表达的影响及其分子机制。方法:将Wistar大鼠随机分为对照组、烟雾组、罗氟司特组、烟雾+罗氟司特组。对照组和罗氟司特组大鼠置于无烟环境中饲养,烟雾组和烟雾+罗氟司特组大鼠暴露于有烟环境,免疫组化法检测各组大鼠肺组织MUC5AC的表达,PCR法检测MUC5AC mRNA表达量,WesternBlot检测MUC5AC蛋白的表达情况。结果:与对照组相比,烟雾组大鼠肺组织MUC5AC的mRNA及蛋白表达水平明显升高(P<0.05),而烟雾+罗氟司特组MUC5AC的表达水平较烟雾组显著降低(P<0.05)。结论:磷酸二酯酶-4抑制剂罗氟司特通过抑制吸烟鼠肺组织MUC5AC的表达,改善COPD。

Objective： To investigate the effect of phosphodiesterase-4 inhibitor roflumilast on MUC5AC expression in smoking rat lung tissue and its molecular mechanism. Methods： Wistar rats were randomly divided into control group, smoking group, roflumilast group, smoking roflumilast group. Control group and roflumilast group rats were placed in smoke-free environment, while, rats in smoking group and smoking with roflumilast group were exposed to smoke environment. Immunohistochemical method was used to detect the MUC5AC expression in lung tissue of each group. The MUCSAC mRNA expression was measured by RT-PCR, the protein expression of MUC5AC was measured by Western Blot. Results： As compared with the control group, the expression level of MUC5AC mRNA and protein were significantly increased in lung tissue of smoking group, While, the expression level of MUC5AC in smoking roflumilast group was reduced significantly as compared with the smoking group. Conclusion： phosphodiesterase-4 inhibitor roflumilast can reverse the smoking induced MUC5AC expression in rat lung tissue and improve COPD.