插入式E-test检测非发酵糖菌药物敏感试验的研究

Insert E-test detection research about nonfermenting bacteria drug susceptibility testing

目的观察一种新型的梯度浓度药敏试条检测临床分离的非发酵糖革兰阴性杆菌对常用药物的敏感性。方法应用8种抗生素的插入式E-test检测78株临床分离的革兰阴性非发酵菌的最小抑菌浓度(MIC)同时应用E-test纸条进行对比。结果标准符合率分别是庆大霉素96.97%、阿米卡星96.97%、头孢他啶96.15%、亚胺培南98.48%、氨曲南98.48%、左氧氟沙星96.15%、复方新诺明94.87%、诺环素98.72%。结论插入式E-test检测非发酵糖革兰阴性杆菌药物敏感试验与对比方法符合性良好,可以满足临床对非发酵菌的MIC检测需要。

Objective To observe the sensitivity of a new type concentration gradient drug sensitive test detecting drug sensitivity of isolated nonfermenting Gram-negative bacilli. Methods Total of 8 antibiotics inserted E-test was used for the detection of the minimum inhibitory concentration （MIC） of 78 clinical isolateed Gram-negative bacilli strains of non-fermentative bacteria, and E-test strips were used for control. Results Standards consistent （CA） rates were as follows： gentamycin 96.97%, amikacin 96.97%, ceftazidime 96.15%, imipenem 98.48%, aztreonam 98.48%, left-ofloxacin 96.15%, compound sulfamethoxazole 94.87%, minocycline 98.72%. Conclusion Inserted E-test to detect the nonfermenting gram-negative bacilli drug sensitivity test and comparison of methods that are good, you can meet clinical needs for MIC testing of non-fermentative bacteria.