油脂酵母Trichosporon fermentans HWZ004的分批补料法高密度培养

Fed-Batch Culture of High Cell-Density Oleaginous Yeast Trichosporon fermentans HWZ004

为提高发酵性丝孢酵母Trichosporon fermentans HWZ004发酵产油脂的能力,采用分批补料的培养方式对其进行高密度培养,考察了初始糖浓度对发酵性丝孢酵母生长及油脂积累的影响,确定初始糖浓度为100 g/L,在该浓度下Trichosporon fermentans HWZ004可获得较高的生物量及油脂产量,且不存在底物抑制现象;然后,在5 L发酵罐中进行分批补料培养,发酵132h后,Trichosporon fermentans HWZ004的生物量、油脂含量及油脂生产强度分别为102g/L、48%和0.37g/(L·h).气相色谱分析结果表明,所得油脂的脂肪酸组成主要为硬脂酸、棕榈酸、油酸、亚油酸,不饱和脂肪酸含量达65%以上,适用于生物柴油的生产.

In order to improve the ability of oleaginous yeast Trichosporon fermentans HWZ004 to produce lipid,a fed-batch culture with high cell density was carried out.The effect of the initial sugar concentration on the growth and lipid accumulation of T.fermentans HWZ004 was investigated and the initial sugar concentration was determined as 100 g/L,at which T.fermentans HWZ004 could achieve more biomass and higher lipid yield without substrate inhibition.After a 132h fed-batch culture in a 5-L fermentor,it is found that the biomass,lipid content and lipid productivity of T.fermentans HWZ004 are respectively 102g/L,48.3％ and 0.37g/（L · h）.GC results reveal that the fatty acids in the obtained lipid are mainly stearic acid,palmitic acid,oleic acid and linoleic acid,and the unsaturated fatty acid content is over 65％,which means that the lipid is suitable for the biodiesel production.