水稻Pi-ta启动子的克隆及其功能分析

Cloning of Pi-ta Promoter and its Function Analysis in Rice

本研究分离了水稻品种日本晴Pi-ta基因的启动子,并构建其GUS融合表达载体,通过农杆菌介导的遗传转化获得了其转基因植株。以获得的转基因水稻为材料进行GUS组织染色和GUS(β-葡萄糖苷酸酶)酶活性分析,结果表明,GUS基因在根、茎和叶中都有表达,转基因植株在接种稻瘟病菌后GUS活性显著增加。用抗病信号分子水杨酸(SA)和茉莉酸甲酯(MeJA)处理后,Pi-ta启动子驱动的GUS活性也显著增加。以上结果表明,Pi-ta启动子是一个对稻瘟病菌应答的诱导型启动子,同时Pi-ta启动子还受到SA和MeJA的诱导表达。

The promoter of Pi-ta in japonica variety of Nipponbare was cloned and its fusion expression vector of GUS was established. The transgenic rice plants were obtained by an Agrobacterium mediated method. By means of GUS histochemical staining and quantitative essay, GUS activity was analyzed in the transgenic rice, the results showed that GUS gene was expressed in root, stem and leaves. GUS activity was increased by inoculation of Magnaporthe oryzae （M. oryzae）. Moreover, GUS activity was also increased by treatment with the resistance-related signaling molecules salicylic acid （SA） and methyl jasmonic acid （MeJA）. Our results indicated that promoter of Pi-ta could respond to M. oryzae as an inducible promoter, as well as to SA and MeJA.