披针叶黄华愈伤组织培养中激素优化组合的研究

Study on the Optimum Combinations of Plant Hormone in Callus Culture of Thermopsis lanceolata

目的:探索不同激素组合对披针叶黄华愈伤组织诱导和增殖的影响。方法:以下胚轴为外植体,以MS为基本培养基,附加2,4-D、BA、NAA和IAA 4种植物激素,采用正交试验研究不同激素配比对披针叶黄华愈伤组织诱导和继代的影响。结果:愈伤组织诱导的最适培养基为MS+2,4-D(0.5 mg/L)+BA(1.0 mg/L)+NAA(1.0mg/L)+IAA(0.5 mg/L),各激素的影响程度为2,4-D>NAA>BA>IAA;愈伤组织继代的最适培养基为MS+2,4-D(0.1 mg/L)+BA(0.1 mg/L)+NAA(0.2 mg/L)+IAA(0.5 mg/L),各激素的影响程度为BA>2,4-D>IAA>NAA;愈伤组织在最优组合的继代培养基上的生长曲线呈"S"型。结论:首次成功建立披针叶黄华愈伤组织快速培养体系,为利用现代生物技术方法保护和利用披针叶黄华物种资源提供了一种新途径。

Objective：To explore the effects of different hormone combinations on callus induction of Thermopsis lanceolata. Methods ： The effects of different hormone concentrations and ratio on callus induction and subculture multiplication of Thermopsis lanceolata were investigated by orthogonal design methods. Results ：The optimal medium of callus induction for hypocotyls explants was MS ＋ 2, 4-D （0. 5 mg/L） ＋ BA（ 1.0 mg/L） ＋ NAA（ 1.0 mg/L） ＋ IAA（0. 5 mg/L）. The best medium of callus subculture multiplication for hypocotyl explants was MS with 2,4-D（0. 1 mg/L） ＋ BA（0. 1 rag/L） ＋ NAA（0. 2 mg/L） ＋IAA （0. 5 mg/L） . In the process of callus subculture multiplication, the effect extent of four kinds of hormones for hypocotyl explants was BA 〉 2,4-D 〉 IAA 〉 NAA. The growth curve of callus culture optimal hormone combination was ＂S＂ curve. Conclusion： The rapid callus culture system for Thermopsis lanceolata is established for the first time. It provides a new way for protecting and utilizing Thermopsis lanceolata species resources through the modern biotechnology.