绿原酸对缺氧环境下PC12细胞活性及缺氧诱导因子1α表达的影响

Effects of Chlorogenic Acid on the Viability and HIF-1α mRNA Expression of PC12 Cells Exposed to Hypoxia

目的:研究绿原酸对缺氧环境下PC12细胞活性及缺氧诱导因子-1α表达的影响。方法:用三气培养箱建立PC12细胞缺氧模型,观察绿原酸对缺氧环境下培养的PC12细胞形态、细胞活力、LDH活性的影响、实时荧光定量PCR检测HIF-1α的基因表达。结果:与缺氧模型组比较,绿原酸可显著提高缺氧PC12细胞存活率、降低LDH活性、改变细胞周期、提高HIF-1α基因的表达水平。结论:绿原酸能提高缺氧PC12细胞的活性并影响缺氧诱导因子1α的表达。

Objective ：To investigate the effects of chlorogenic acid on the viability and HIF-la mRNA expression of PC12 cells exposed to hypoxia. Methods ：PC12 cells were cultured in trigas incubator in order to establish the hypoxie condition. The effects of ehlorogenic acid on the cells were evaluated by morphological observation, cell viability and LDH release assays as well as the examination of mRNA expression level of HIF-1α. Results： Chlorogenic acid significantly improved the viability of cells exposed to hypoxia, decreased LDH release,arrested the cell cycle on G1 phase,and increased the gene expression level of HIF-1α. Conclusion：Chlorogenic acid protects PC12 cells from hypoxic damage by improving the expression of HIF-1α.