宽心解郁汤联合有氧运动抗抑郁效果的实验研究

Study on Antidepressant Effect of Kuanxinjieyutang Combined Aerobic Exercise

目的:观察宽心解郁汤、短期有氧运动及二者联合干预的抗抑郁作用,探讨可能机制。方法:SPF级雄性昆明小鼠,随机分成空白对照组、丙咪嗪阳性对照组、宽心解郁汤低剂量组(生药1.8 g/kg)、宽心解郁汤高剂量组(生药3.6 g/kg)、运动组(游泳30 min/d)、联合组(宽心解郁汤低剂量联合运动),干预8 d;通过强迫游泳、悬尾实验以及高架十字迷宫实验评价抗抑郁效果;处死小鼠,ELISA法检测脑组织单胺类神经递质含量,Western blotting分析脑组织BDNF表达。结果:宽心解郁汤高剂量表现出与丙咪嗪相似的抗抑郁效果,而低剂量抗抑郁效果不显著。单独的运动有与丙咪嗪相似或更好的抗抑郁效果。联合组疗效最佳。与空白对照组比较,宽心解郁汤高剂量组脑组织NE、DA含量以及运动组脑组织NE、DA及5-HT含量显著升高,丙咪嗪组、宽心解郁汤高剂量组、运动组及联合组脑组织BDNF蛋白表达显著高于空白对照组,运动组、联合组脑组织BDNF蛋白表达高于宽心解郁汤低、高剂量组,联合组脑组织BDNF蛋白显著升高。联合组脑组织NE、DA及5-HT含量显著高于阳性对照组。结论:适量宽心解郁汤、短期有氧运动以及联合干预对轻度抑郁有显著的抗抑郁疗效,其机制可能与增加脑组织NE、5-HT含量,引起脑组织BDNF蛋白表达增多,进而影响神经再生及可塑性、改善认知功能有关。

Objective：To observe antidepressant effects of Kuanxinjieyutang （KXJYT） , short-term aerobic exercise and their joint intervention and its possible mechanism. Methods：Ninty-six SPF male KM mice were randomly divided into control group,imipramine, low dose （ KXJYT 1.8 g crude drugs/kg bw/time/day）, high dose （3.6 g crude drugs/kg bw/time/day） , exercise group （ swimming 30 rain/times/day） ,joint group （low dose and swimming ）, with 8 days intervention. The antidepressant effect was evaluated by forced swimming,tail suspension test and overhead cross maze test. After mice were killed, brain monoamine neurotransminer levels of mice were detected by ELISA, and BDNF expression in brain was analyzed by Western blotting. Results ： High dose group showed antidepressant effect like imipramine while low dose didn＇t. Exercise group showed similar or better antidepressant effect than imipramine. Joint group showed better interaction antidepressant effect. Brain NE, DA of high dose group and brain NE, DA and 5-HT content of exercise group increased significantly, similar to imipramine. Brain NE, DA and 5-HT content of Joint group were significantly higher than those of imipramine. The protein expression of BDNF in brain was more in imipramine, high dose, exercise and joint groups than that of control group, sports group was higher than that of low dose and high dose group ,joint group was obviously higher than other groups. Conclusion ： Moderate KXJYT, short-term aerobic exercise and their joint intervention have obvious antidepressant effect on mild depression. Its mechanism may be related to increasing brain NE and 5-HT content, and inducing brain BDNF expression, which may affect the nerve regeneration and plasticity, improve cognitive function.