响应面分析法优化大叶千斤拔中染料木素提取工艺

Optimal Extraction Technology for Genistein in Flemingia macrophylla with Response Surface Method

目的:采用响应面分析法优化大叶千斤拔中染料木素的提取工艺。方法:在单因素试验基础上,以提取温度、提取时间、溶媒比为响应因子,染料木素的提取率为响应值,通过对响应因子各水平的多元线性回归及二项式拟合,用响应面法选取较佳工艺,并进行预测分析。结果:各因素对染料木素提取率的影响大小依次为提取温度>提取时间>溶媒比。确定最佳提取工艺条件为提取温度80℃,提取时间3.5 h,溶媒比20 mL/g,在此条件下,大叶千斤拔中染料木素提取率为0.161 mg/g。结论:试验结果与模型预测值相符,该工艺可用于大叶千斤拔中染料木素的提取。

Objective：To optimize extraction techniques for genistein in Flemingia macrophylla by using response surface method. Methods： Based on the single factor experiment, extraction temperature, extraction time and solvent ratio served as the response factor while the extraction rate of genistein served as the response value. Based on multiple linear regression and binomial expression, response surface method was employed to optimize the extracting process, and predicative analysis was conducted. Results ： The effects of factors in the descending order were as follows： extraction temperature, extraction time and solvent ratio. The optimal extraction technology was as follows： extraction temperature 80 ℃, extraction time 3.5 h and solvent ratio 20 mL/g. Under these conditions, the extraction rate of genistein reached 0. 161mg/g. Conclusion： The results are agreed with model predictions. The technology can be used as the extraction process of genistein in Flemingia macrophylla.