固相萃取-高效液相色谱法测定人血浆中对乙酰氨基酚

Determination of paracetamol in human plasma by solid phase extracted HPLC method

目的 :建立一快速、灵敏的对乙酰氨基酚 (PAC)体内浓度的测定方法 ,并用于其生物利用度的研究。方法 :以茶碱为内标 ,采用固相萃取的 HPL C分析法 ,取样 0 .2 m l。YWG- C1 8色谱柱 ,流动相为甲醇 /乙腈 /水 (2 5 /15 /36 0 ,V/V) ,流速 1.0 ml/min,检测波长 2 5 4nm。结果 :PAC的最低检测浓度为 0 .0 5︼g/m l。线性范围 0 .2 0～ 15 .0︼g/ml。平均批内、批间 RSD均 <6 % ,方法回收率接近 10 0 % ,RSD<5 %。结论 :本方法快速灵敏、干扰少、重现性好 ,符合 PAC生物利用度研究的要求。

AIM: To establish a new high performance liquid chromatographic (HPLC) method for determination of paracetamol in human plasma, and apply to study of the relative bioavailability of paracetamol. METHODS: Aminophylline was used as internal standard (IS). Plasma (0 2 ml) was extracted by a C 2 solid phase extraction (SPE) cartridge. Mobile phase was methanol: acetonitrile: water (25∶15∶360). RESULTS: The minimum detective concentration of paracetamol was 0 05 μg/ml, and concentrations range of standard curve was from 0 20 μg/ml to 15 0 μg/ml. Average within run precision and between run precision were near 6%. Recoveries of the method were almost 100%, with relative standard deviation (RSD)<5%. CONCLUSION: The method can be applied to study the relative bioavailability of paracetamol.