高效液相色谱法测定紫草油中β,β'-二甲基丙烯酰阿卡宁的含量

Determination of β,β'-dimethylaerylakannin in Shikonin Oil by HPLC

目的：建立紫草油中β，β′-二甲基丙烯酰阿卡宁的含量测定方法。方法：采用高效液相色谱（HPLc）法，色谱柱为迪马C18钻石色谱柱（250mm×4．6mm，5μm）；流动相为乙腈．水-甲酸（75：25：0．2）；检测波长为275nm；柱温为室温。结果：在80．6～564．5ng范围内，β，β′-二甲基丙烯酰阿卡宁进样量和峰面积具有良好的线性关系，线性回归方程为C=1．0279×10^-6A＋2．3589×10^-2，r=0．9999。测得紫草油中β，β′-二甲基丙烯酰阿卡宁平均含量为0．8692mg/ml。结论：该方法简便可行，重现性好，可作为紫草油的质量控制方法。

OBJECTIVE：To establish a method for determination of β,β′-dimethylaerylakannin in Shikonin oil. METHODS： The sample was determined by HPLC and separated on chromatographic column Diamonsil C18 （250mm×4.6mm,5μm） with mobile phase consisting of Acetonitril-H2O-HCO2H（75：25：0.2） at detection wavelength of 275 nm. The column temperature was kept at room temperature. RESULTS： The linear range ofβ,β′-dimethylaerylakannin was 80. 6-564. 5 ng, and the linear equation was as follows： C = 1. 027 9 ×10^-6A ＋ 2. 358 9 ×10^-2（r = 0. 999 9）. The average content ofβ,β′-dimethylaerylakannin in Shikonin oil was 0. 869 2 mg/ml. CONCLUSION： The method is simple and reproducible and it can be used as the quality control method for Shikonin oil.