摘要
目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。
Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第1期45-47,共3页
Chinese Journal of Zoonoses
基金
福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0 )
