几种幽门螺杆菌检测方法比较

COMPARISON OF THE DIFFERENT DETECTIVE METHODS FOR HELICOBATER PYLORI

目的 探讨 4种幽门螺杆菌 (Hp)检测方法的差别及其临床价值。 方法 采用斑点金免疫渗滤法 (DIGFA)检测患者末梢血及唾液中的抗HP -IgG抗体 ,同时用快速尿素酶试验 (RUT)、PCR -HP -DNA检测胃粘膜活检组织中的Hp -IgG感染情况。结果 10 0例患者Hp检测阳性率分别是 :末梢血抗Hp -IgG法 84% ,唾液抗Hp -IgG法 5 8% ,RUT5 9% ,PCR -Hp -DNA法 6 2 % ,几种方法诊断Hp现症感染的准确性分别为 72 .72 %、90 %、91%、92 % ;敏感性分别为 94.6 4%、85 .71%、87.5 0 %、94.6 4% ;特异性分别为 35 .2 9%、97.0 6 %、97.0 6 %、91.18% ;阳性预测值分别为 :70 .6 7%、92 .31%、92 .45 %、94.6 4% ;阴性预测值分别为 80 .0 0 %、86 .84%、89.19%、91.18%。结论 DIGFA法检测唾液中Hp -IgG检测Hp现症感染的准确性、敏感性、特异性与胃粘膜RUT相近 ,为非侵入性、简便、快速、价廉的方法 ,适合于临床推广。血抗Hp -IgG检测适合于流行病学调查 ;PCR -Hp

Aim To explore the differences and clinic value of the different detective methods for Helicobacter pylori(Hp). Methods Detecting anti-Hp-IgG antibody in blood and saliva of patients with DIGFA.Decting Hp infection in biopsy specimen of gastric mucosa with RUT and PCR-Hp-DNA at the same time.Results The anti-Hp-IgG antibody in blood, anti-Hp-IgG antibody in saliva, RUT in gastric mucosa and PCR-Hp-DNA in gastric mucosa, the positive rate was 84%, 58%, 59%, 62%, respectively, while the correct rate for diagnosing Hp infection is 72.72%, 90%, 91%, 92%.The sensitivity is 94.64%, 85.71%, 87.50%, 94.64%. The specificity was 35.29%, 97.06%, 97.06%,91.18%. The positive predictive rate was 70.67%, 92.31%, 92.45%, 94.64%. The negative predictive rate is 80.00%, 86.84%,89.19%, 91.18%. Conclusions The correct rate, sensitivity and specificity of anti-Hp-IgG in salviva with DIGFA are similar to gastric mucosa with RUT. Both of these two methods are non-invasive, simple, rapoid, cheap and adaptable to clinic test. Anti-Hp-IgG antibody in blood is suitable for epidemic investigation. PCR-Hp-DNA is suitable for scientific researches.