脂多糖诱导大鼠DIC模型水通道蛋白5的表达与肺水肿的关系

The Relation of Aquaporin 5 Expression and Lung Edema in Rats Disseminated Intravascular Coagulation

目的研究对Wistar大鼠滴注LPS造成DIC后，肺细胞膜表面水通道蛋白5（AQP5）的表达变化与肺水肿的关系。方法清洁级，雄性Wistar大鼠随机分为：对照组（滴注生理盐水组）；实验组（滴注LPS后4h、6h、8h、10h组）。各组取血检测PLT计数、PT、APTT、FIB含量、DD水平；取各组大鼠左肺测量其肺湿／干重比值；取各组肺组织进行HE染色，观察肺组织中微血栓形成情况及组织病理损伤（结合血液学指标与组织病理损伤判断DIC病程）；提取大鼠肺组织细胞总RNA，RT-PCR方法检AQP5mRNA水平的表达。采用SNK—q及直线相关方法对结果进行统计学分析。结果各实验组PLT计数、PT、APTT、FIB含量、DD水平与对照组相比P〈0．01；滴注LPS后6h在肺组织中可见微血栓，滴注LPS后8h、10h肺组织结构紊乱，肺泡中有大量渗出液；滴注LPS后4hAQP5的mRNA表达下调，与对照组相tLP〈0．01；滴注LPS后4h～10h肺湿／干重比值增加。经直线相关分析肺湿／干重比值与AQP5的mRNA呈负直线相关，P〈O．01。结论DIc时肺水肿的程度与肺细胞表面AOP5的表达相关．有望通过调节肺细胞表面AOP5的表达干预DIG时肺水肿的形成或消除。

Objective To study the relation between the change of lung Aquaporin 5（AQP5） and lung edema in Wistar rats,in which DIC emerged after dripping LPS.Methods Fifty male Wistar rats（clean grade） were randomly divided into 5 groups（10 per group）：the control group,LPS （sustained 4h infusion of 30mg/kg via rat vena caudalis） 4h,6h,8h and 10h groups.PLT count,PT, APTT, levels of FIB and DD of blood were detected;pathological changes of each group＇s lung tissues were examined by HE dyeing（both hematology parameters and tissues pathological changes were used to judge the course of DIC）;each group＇s lung tissue RNA were extracted and RT-PCR were used to detect the mRNA expression of AQP5.Statistical analysis was performed using SNK-q and linear correlation analysis.Results Each experiment group＇s PLT count,PT, APTT, levels of FIB and DD were compared with control group（P〈0.01）;and microthrombus can be detected in lungs in LPS 6h group,lung tissues＇ structure were destroyed and much effusion in alveolus in LPS 8h and 10h groups;the expression ofAQP5 mRNA appeared down regulation in LPS 4h group compared with control group（P〈0.01）;in LPS 4h group lung wet/dry weight increased compared with control group（P〈0.01）,after that lung wet/dry weight increased obviously.The extent of lung edema and the expression of AQP5 were linear correlation（P〈0.01）.Conelusion The extent of lung edema and the expression ofAQP5 were linear correlation in rats DIC,it is hopeful to interfere the formation and remove lung edema through adjusting the expression ofAQP5.