摘要
目的：观察阿尔茨海默病（AD）大鼠皮质神经元中溶酶体蛋白酶Cathepsin D的表达。方法采用β-淀粉样肽（Aβ）大鼠海马注射制作AD动物模型，用免疫组织化学染色和Western-blot印迹法检测大鼠颞底皮层Cathepsin D的表达。Y迷宫检测大鼠空间辨别性能和学习记忆能力。TUNEL法检测细胞凋亡情况。结果Aβ脑池内灌注造成Aβ沉积的AD模型在行为学和病理学改变上一定程度地模拟了AD。免疫组化和Werstern-bolt检测均显示实验组Cathepsin D阳性神经元数量较假手术组和正常组明显增加（P＜0.05）。TUNEL法检测显示AD大鼠颞底皮层神经元凋亡与正常组和假手术组相比显著升高（P＜0.05）。结论Cathepsin D在AD大鼠皮层脑组织中表达升高，可能参与了神经元凋亡等病理过程。
Objective To observe the Alzheimer’s disease（AD） rat cortical neurons in lysosome protease Cathepsin D expression.Methods Using amyloid-beta peptide（Aβ） animal model of rat hippocampal injection production AD with immunohistochemical staining and Western blot imprinting method to detect the expression of Cathepsin D end of temporal cortex in rats,and Y maze to test spatial discrimination performance and the ability of learning and memory in rats.TUNEL method to detect apoptosis.Results Caused A beta deposition pool A beta brain perfusion of AD model on behavioural and pathology change part to simulate the AD.Immunohistochemical and Werstern-bolt test showed that the experimental group,Cathepsin D positive neuron numbers significantly increased compared with control group and normal group（P〈0.05）.TUNEL method showed that the bottom of the AD rats with temporal cortex neuron apoptosis signiifcantly increased compared with normal group and control group（P〈0.05）.Conclusion Cathepsin D expression increased in brain tissue in AD rats cortex,may participate in the pathological process of neurons apoptosis.
出处
《中国血液流变学杂志》
CAS
2013年第3期435-437,556,F0003,共5页
Chinese Journal of Hemorheology
