氧化电位水对HBsAg破坏效果的试验及临床观察

Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water

目的 观察氧化电位水 (EOW)在胃镜消毒中对乙型肝炎表面抗原 (HBsAg)抗原性的破坏效果。方法 模拟现场试验用EOW与人工污染的胃液作用 1min ,观察其对HBsAg的破坏作用 ,以PBS作为对照组。现场试验以HBsAg阳性病人用后的污染胃镜 ,作为消毒前对照 ,经去有机物处理及EOW浸泡 1min ,胃镜表面涂抹采样 ,用ELISA检测HBsAg。结果 人工污染胃液 (HBsAg平均S/N值为 42 .16 )加入胃液量 10 0倍的EOW作用 1min后 ,HBsAg检测为阴性 ,对照组仍为阳性。临床污染胃镜 (HBsAg平均S/N值为5 .99)经清洁、消毒后HBsAg检测为阴性。 结论 在清除有机物后 。

Objective Observation on the effect of disinfection on gastroscope, contaminated by hepatitis B surface antigen (HBsAg) in the electrolyzed oxidizing water(EOW). Methods Contaminated gastric juice and serum was added to EOW for 1 minute. Positive control samples were treated with PBS instead of EOW in the same way. Gastroscopes used for hepatitis patients were immersed in the EOW for 1 minute after cleaning. Samples were collected before and after treatment. ELISA was used to test HBsAg. Results With mixed samples (average S/N= 42.16 ) of EOW, HBsAg became negative when diluted in 100 times. However, the HBsAg of positive control samples remained positive. After cleaning the gastroscope (average S/N= 5.99 ) immersed in EOW, HBsAg became negative. Conclusion EOW was effective in destroying HBsAg which could be used for gastroscope disinfection.