高效液相色谱法测定糖果制品中喹啉黄、专利蓝V、新胭脂红和固绿

Determination of ouinline yellow,patent blue v,new coccined and fast green FCF by high performance liquid chromatograph in candies.

建立了糖果制品中喹啉黄、专利蓝V、新胭脂红和固绿的高效液相色谱测定方法。样液经聚酰胺粉吸附,用甲醇-甲酸混合溶液洗涤,用乙醇-氨水-水溶液解吸附,浓缩后加水定容后上机测试。使用Agilent XDB C18色谱柱,以甲醇和0.02mol/L乙酸铵为流动相梯度洗脱,外标法定量。结果表明,喹啉黄、专利蓝V、新胭脂红和固绿在5 80μg/mL范围内线性关系良好,相关系数为0.99973 0.99999,加标回收率为90.6%100.9%,RSD为2.54%5.01%,方法检出限为0.20 0.50mg/kg。该方法简便、准确、高效,可用于糖果制品中喹啉黄、专利蓝V、新胭脂红和固绿的测定。

To establish a method for the determination of Ouinline yellow,Patent blue V,New Coccined and Fast green FCF in candies by HPLC.Samples were extracted by polyamide,eluted by methyl-methanoic acid and adsorbed and desorbed by ethanol-ammonia-water solution.An Agilent XDB C18 column was used for chromatographic separation,methanol-0.02 mol/L ammonium acetate solution was the mobile phase.The linear range of the developed method was 5 ～ 80μg/mL with correlation coefficients from 0.99973 to 0.99999.The recovery of the method was from 90.6％ to 100.9％,and the relative standard deviation （RSD） was from 2.54％ to 5.01％.The detection limit was from 0.20 to 0.50 mg/kg.This method is simple,accurate and suitable for the determination of Ouinline yellow,Patent blue V,New Coccined and Fast green FCF in candies.