高效液相色谱法测定蜂胶软胶囊类保健食品中吡啶甲酸铬

Determination of chromium picolinate in propolis soft capsule by high performance liquid chromatography

吡啶甲酸铬能增强胰岛素活性,改善人体糖代谢,是一种被卫生部所允许的、能添加到保健食品中的功效成分,已被添加于许多标示具有调节血糖功能的蜂胶软胶囊类保健食品中。蜂胶软胶囊类保健食品成分复杂多样,除蜂胶外,还含有蜂蜡、色拉油、明胶、卵磷脂等。通常使用的保健食品中吡啶甲酸铬含量的测定方法不能满足此类样品的要求。针对这一问题,建立了蜂胶软胶囊类保健食品中吡啶甲酸铬含量的高效液相色谱测定方法。样品采用四氢呋喃/甲醇/水体系提取,Phenomenex PFP色谱柱分离,梯度洗脱,二极管阵列检测器检测,峰面积外标法定量。吡啶甲酸铬在1 150 mg/L范围内线性关系良好,定量限(LOQ)为50 mg/kg,方法回收率为89.2%98.3%,相对标准偏差(n=6)为1.1%1.9%。此方法适用于蜂胶软胶囊类保健食品中吡啶甲酸铬含量的测定。

Chromium picolinate is a functional ingredient permitted to use in health food by Ministry of Health,which can enhance the activity of insulin and improve the sugar metabolism in the human body.It has been added in propolis soft capsules marking function of regulating blood sugar.Ingredients of propolis soft capsules are complex and diverse,including not only propolis but also beeswax,salad oil,gelatin,lecithin and so on.Determination methods usually used for chromium picolinate in health food are not adequate for propolis soft capsules.For the problem,an high performance liquid chromatography method was developed for determination of chromium picolinate in propolis soft capsule.Chromium picolinate was extracted by tetrahydrofuran/methanol/water system,seperated by Phenomenex PFP column using gradient elution and detected by diode array detector.Quantitative analysis was based on the peak area by external standard method.Good linear relationship was achieved in the concentration range of 1 ～ 150 mg/L for chromium picolinate.The LOQ level for chromium picolinate was 50 mg/kg,and recoveries were 89.2％ ～ 98.3％,with relative standard deviations of 1.1％～ 1.9％.The method can be used for determination of chromium picolinate in propolis soft capsule.