胎儿、新生儿、儿童及成人角膜T细胞和细胞间黏附分子的检测

T cell and molecule-1 detection in cornea of fetus, neonates, children and adults

目的 探讨不同年龄人角膜的免疫状态。方法 采用免疫组化法 ,用 5种单克隆抗体对不同年龄的人角膜切片进行免疫组化染色 ,观察人角膜的免疫状态。结果 正常人中 ,成人角膜T细胞 (CD+3 )、辅助 /诱导性T细胞 (CD+4 )、抑制 /细胞毒T细胞 (CD+8)、巨噬细胞 (CD+68)及细胞间黏附分子 1(CD+54 )含量较少 ,主要分布于角膜缘 ,角膜中央区未见。胎儿、新生儿及儿童角膜中上述阳性细胞较成人少。结论必要时胎儿、新生儿及儿童角膜可作为供体角膜用于角膜移植术。儿童角膜移植术后可减少免疫抑制剂的用量。

Objective To study the immune state of human cornea with different ages Methods The human corneas in different ages were detected by using immunohistochemical techniques for 5 types of monoclonal antibody Results The contents of T cell (CD + 3), helper/inducer (CD + 4), toxic T cell (CD + 8), macrophage (CD + 68 ) and molecule 1 (intercellular adhesive molecule, CD + 54 ) in the adult cornea were relatively low, mainly in the limbus but none at the corneal center These positive cells were less in corneas of fetus, neonates and children than that in adults Conclusion In corneas of fetus, neonates and children, the T cell, macrophage and molecule 1 were less than that of adults Their corneas may be used as donors in corneal transplantation