摘要
目的:采用PLGA-PEG为聚合材料,制备RGD修饰包载超顺磁性四氧化三铁纳米粒子(RGD-NP-Fe3O4),用于脑胶质瘤细胞靶向核磁共振成像纳米探针。方法:采用沉淀法制备RGD修饰的载超顺磁性纳米粒,考察纳米粒的粒径,电位等理化指标以及细胞毒性。通过细胞以及肿瘤球摄取实验,考察RGD-NP-Fe3O4的脑胶质瘤细胞靶向性。结果:制备得到的RGD-NP-Fe3O4粒径在85±7.5 nm,电位为18±1.15 mV。纳米粒浓度在300μg/mL范围内,对脑胶质瘤细胞均无显著毒性。经过RGD修饰后脑胶质瘤细胞U87对纳米粒的摄取效率大大提高,纳米粒穿透肿瘤球能力显著增强。结论:RGD修饰包载超顺磁性氧化铁纳米粒是一种潜在的高效的脑胶质瘤细胞靶向诊断纳米探针和靶向给药系统。
Objective： To prepare the RGD-NP-Fe3O4 with the materials of PLGA-PEG for the nano probe of the nuclear magnetic resonance imaging for targeted glioma cells. Methods： The nano was prepared by the method of precipitation. The size of the nano particles, the physical and chemical index and the cell toxicity were inspected. The targeting efficiency was evaluated by U87 cellular uptake and tumor spheroids. Results： The particle diameter of RGD-NP-Fe3O4 was 85~ 7.5 nm with the Zeta potential of 18± 1.15 mV. The result demonstrated that the RGD-NP-Fe3O4 could be taken up by U87 with 2.7 times which was higher than that of the NP-Fe3O4. The evaluation of tumor spheroid penetration shows RGD-NP-Fe3O4 has the strongest fluorescence intensity. Conclusions： RGD-NP- Fe3O4 is a potential effective drug delivery system for glioma diagnose and treatment.
出处
《现代生物医学进展》
CAS
2013年第35期6814-6817,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81101604)
