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TGF-β1和Smad4在膀胱癌中的表达及其与淋巴结转移的关系 被引量：3

Correlation of TGF-β1 and Smad4 Expression with Lymph Node Metastasis in Bladder Cancer
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摘要 目的:观察转化生长因子β1(TGF-β1)和Smad4在膀胱癌组织内的表达情况,并分析其与膀胱癌淋巴管生成及淋巴结转移之间的关系。方法:选择在本院确诊的膀胱癌患者50例,根据淋巴结转移与否分为淋巴结转移组(30例)和无淋巴结转移组(20例)。应用免疫组化法检测膀胱癌组织内TGF-β1和Smad4的表达。以D2-40作为淋巴管内皮特异性标记物,检测膀胱癌组织内淋巴管生成情况并分析其与TGF-β1和Smad4的表达的关系。结果:TGF-β1主要表达于膀胱癌细胞胞浆内,在淋巴结转移组的表达率明显高于无淋巴结转移组(P=0.017)。Smad4表达于膀胱癌细胞胞浆和胞核内,在无淋巴结转移组的表达率明显高于有淋巴结转移组(P=0.005)。Smad4表达阳性组的淋巴管数密度(LVD)明显低于Smad4表达阴性组(P=0.037)。TGF-β1的表达与Smad4的表达呈显著的负相关性(P=0.001)。结论:TGF-β1的表达与膀胱癌淋巴管生成及淋巴结转移呈显著正相关,Smad4的表达与膀胱癌淋巴管生成及淋巴结转移呈显著负相关,TGF-β1/Smad4信号通路可能在膀胱癌淋巴管生成和淋巴结转移过程中起重要作用。 Objective： To investigate the correlation of transforming growth factor β1 （TGF-β1） and Smad4 with the lymphangiogenesis and lymph node metastasis of bladder cancer. Methods： Fifty paraffin-embedded specimens from patients with bladder cancer were included in this study.and divided into lymph node metastatic group （n=30） and nonmetastatic group （n=20）. The expressions of TGF-β1 and Smad4 were detected by immunohistochemical stain in bladder cancer. D2-40 was used as the specific lymphatic endotheli- al marker for detecting lymphangiogenesis. Results： TGF-β 1 protein was observed predominantly in the cytoplasm of the tumor cells, the expression of TGF-β1 in lymph node metastatic group was significantly higher than that in nonmetastatic group（P=0.017）. Smad4 protein was observed in cytoplasm and nucleus of tumor cells, the expression of Smad4 in nonmetastatic group was significantly higher than that in lymph node metastatic group（P=0.005）. The lymphatic vessel density（LVD） in Smad4 positive group was significantly decreased than that in Smad4 negative group （P=0.037）. TGF-[31 expression was negatively correlated with Smad4 expression in bladder cancer（P=0. 001）. Conclusion： TGFq31 expression was positively correlated with lymphagiogenesis and lymph node metastasis, whereas Smad4 expression showed negative correlation with lymphangiogenesis and lymph node metastasis in bladder cancer, which indicated that TGF-β1/Smad4 signal pathway may play an important role in the lymphangiogenesis and lymph node metastasis of bladder cancer.
作者 李建章 王莉 范准 杨树才 李鑫磊 马晶
机构地区 哈尔滨医科大学附属第四医院泌尿外科 哈尔滨医科大学解剖教研室
出处 《现代生物医学进展》 CAS 2013年第35期6860-6863,6885,共5页 Progress in Modern Biomedicine
关键词 转化生长因子Β1 SMAD4 淋巴结转移 淋巴管生成 膀胱癌 TGF-β1 Smad4 Lymph node metastasis Lymphangiogenesis Bladder cancer
分类号 R737.31 [医药卫生—肿瘤]
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参考文献20

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二级参考文献11

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现代生物医学进展
2013年 第35期

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