NFAT1和TWEAK在宫颈鳞癌及上皮内瘤变中表达的意义

Significance of NFAT1 and TWEAK Expression in Cervical Intraepithelial Neoplasia and Squamous Cell Carcinoma

目的:通过观察NFAT1和TWEAK在正常宫颈鳞状上皮、宫颈鳞状上皮上皮内瘤变、宫颈鳞癌中表达,探讨NFAT1和TWEAK表达情况在宫颈鳞癌发生和临床发展中的意义。方法:研究组织病例为档案病理蜡块,应用免疫组织化学技术检测NFAT1和TWEAK的表达;对宫颈鳞癌进行临床分期,分析NFAT1和Tweak表达与临床分期的相关性。结果:免疫组化结果显示,NFAT1阳性表达率在正常宫颈鳞状上皮、CINⅠ级、CINⅡ级、CINⅢ级、鳞癌中分别为24.4%,24.5%,29.2%,72.7%,78.4%,各组总体阳性表达率存在显著性差异,CINⅢ级与宫颈鳞癌中NFAT1表达率较宫颈正常上皮,CINⅠ级,CINⅡ级与CINⅢ级显著增高。TWEAK阳性表达率在正常宫颈鳞状上皮、CINⅠ级、CINⅡ级、CINⅢ级、鳞癌中分别为73.4%,66%,73.1%20.4%,19.6%,各组总体阳性表达率存在显著性差异。宫颈正常,CINⅠ级,CINⅡ级与CINⅢ级较CINⅢ级与宫颈鳞癌中TWEAK表达率显著增高。NFAT1和TWEAK表达相关性具有统计学意义。结论:NFAT1和TWEAK在正常宫颈上皮、宫颈上皮内瘤变、宫颈鳞癌中的表达存在差异且两者间表达存在相关性,NFAT1表达增加与宫颈鳞癌发生和肿瘤进展有关而TWEAK表达与宫颈癌发生呈负相关。

Objective： Significance of NFAT 1 and TWEAK expression in development and progression of cervical carcinoma will be investigated after NFAT1 and TWEAK expression were observed in normal cervical epithelium, cervical intraepithelial neoplasias, cervical carcinoma. Methods： Cervix tissue were collected NFAT1 and TWEAK expression were assayed by immunohistochemical method, and relation of NFAT1 and TWEAK expression to clinical stage was analysized. Results： Immunohistochemical results showed that NFAT1 positive signal located in cell Nucleus, its expression positive rate were 24.4%, 24.5%, 29.2%, 72.7% and 78.4% in normal cervical epithelium, cervical intraepithelial CIN I, C1N II, C1NIII, cervical carcinoma. NFAT1 expressed significantly higher in C1NIII and cervical carcinoma than others. Expression positive rate of TWEAK were 73.4%, 66%, 73.1%, 20.4% and 19.6%, in normal cervical epithelium, cervical intraepithelial CIN I , C1N II, CINTII, cervical carcinoma. TWEAK expressed significantly lower in C1N III and cervical carcinoma than others. Further analysis showed there is significant relation between NFATI and TWEAK expression. Conclusion： There is difference between NFAT1 and TWEAK expression among normal cervical epithelium, cervical intraepithelial neoplasias, cervical carcinoma and there is a significant between two factors. NFAT1. hypo-expression was associated with cervical carcinoma development and there is a negative correlation between NFAT1 expression and cervical carcinogenesis.