兔肢体火器伤骨骼肌组织能量代谢与脂质过氧化物的变化

Changes of energy metabolism and lipid peroxidation in skeletal muscles of rabbit limbs wounded by firearm

目的 探讨软组织火器伤时肌肉组织的能量代谢变化。方法 采用兔肢体火器伤模型 ,测定致伤前后不同区域与不同时间骨骼肌组织的Na+ K+ ATP酶活性变化。结果 致伤平均传递能量为 ( 1 .0 3± 0 .33)J,骨骼肌Na+ K+ ATP酶活性伤后 3h下降 ,6～ 1 2h回升 ,2 4h再次下降 ,ATP含量变化与Na+ K+ ATP酶活性变化呈正相关 ,而骨骼肌丙二醛 (Malondialdehyde ,MDA)含量伤后明显上升。结论 火器伤后 1 2h内进行伤口的初期外科处理 ,是减少伤后骨骼肌能量代谢障碍的最佳时机。

Objective To explore the changes of energy metabolism in skeletal muscles resulting from firearm. Methods The changes of Na + K + ATPase activity in wounded skeletal muscles of different areas and at different time points were determined. Results The average transmission speed of the energy is 1.03±0.33 J/mm from the wound tract to the surroundings. The activity of Na + K + ATPase in skeletal muscles was decreased in 3 h after wound, and recovered in 6 to 12 h, and then declined again in 24 h, which had a positive correlation with ATP content. While the malondialdehyde (MDA) content in the skeletal muscles was increased obviously after trauma. Conclusion The optimal time to manage the firearm wounds should be within 12 hours after the injury so that the energy metabolism dysfunction of skeletal muscles can be minimized.