摘要
目的 :观察透骨草提取物对小鼠肝癌22(H22)细胞生长的抑制作用,进一步探讨透骨草提取物对H22细胞生长抑制的机制。方法:通过皮下接种H22细胞建立荷瘤H22模型,15 d后称取瘤组织重量并按公式计算抑瘤率,免疫组化方法检测H22细胞β-连环蛋白(β-catenin,β-cat)和细胞周期蛋白D1(cyclin D1,Cyc D1)的表达。结果:透骨草提取物对H22细胞的抑瘤率为43.16%,明显高于对照组(P<0.05)。透骨草提取物处理的H22细胞β-cat和Cyc D1的表达明显低于对照组(P<0.05)。结论:透骨草提取物对H22细胞的生长有抑制作用,其机制可能与下调β-cat和Cyc D1的蛋白表达有关。
Objective： To observe the inhibition of phryma leptostachya L . var . asiatica hara extract on the growth of hepatocellular carcinoma H22 cells in mouse and investigate the its inhibiting mechanism on hepatocellular carcino-ma H22 cells . Methods： The tumor-bearing model was established by subcutaneous inoculation of mouse hepatocellu-lar carcinoma H22 cells . After 15 days , the tumor tussues and the tumor inhibitory rate were calculated according to the formula. Immunohistochemistry technique was used to detect the expressions of β-catenin and cyclin D1 on the hepatocellular carcinoma H22 cells . Results： The inhibition rate of phryma leptostachya L . var . asiatica hara extract for the hepatocellular carcinoma H22 cells was 43.16% , which was higher than that of the control group（P〈 0.05）. The expressions of β-catenin and cyclin D1 proteins of the hepatocellular carcinoma H22 cells treated with phryma lep-tostachya L.var.asiatica hara extract were significantly lower than the control group（P 〈 0.05）. Conclusion： Phryma leptostachya L . var . asiatica hara extract can inhibit the growth of mouse hepatocellular carcinoma H22 cells and the mechanism may be associated with the downregulation of the expression of β-catenin and cyclin D1 proteins.
出处
《中国执业药师》
CAS
2014年第1期20-22,27,共4页
China Licensed Pharmacist
