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四川主栽小麦品种RAPD标记遗传差异研究 被引量:21

The Study on Genetic Variation of Sichuan Major Wheat Cultivars by RAPD Analysis
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摘要 采用随机扩增多态性DNA(RAPD) 标记,对四川近50 年来年推广面积6-67 万hm2(100万亩)以上的40 个小麦主栽品种遗传差异进行了初步探讨。结果表明,55 个随机引物中,有32个引物( 占58-2% ) 扩增产物具有多态性。32 个引物共扩增出185 条带,其中93 条带( 占50% )具有多态性,每个引物可扩增出1 ~11 条多态性带,平均2-9 条。40 个品种RAPD 标记遗传距离(GD) 变异为0-019 ~0-475 ,平均GD 值为0-221。聚类分析表明,在GD 值0-23 水平上,40 个品种可聚为5 类。一些随机引物对有些品种能进行特异性扩增。引物OPN14 对小麦1BS 扩增能产生特异性DNA 片段,能完全鉴定出40 个供试品种中的9 个1BL/1RS小麦- 黑麦易位系品种。据此认为,RAPD标记可以作为小麦品种鉴定的指纹图谱。 Genetic variation is the basis for genetic improvement and plant breeding. This study aimed to estimate the genetic variation by RAPD technique among 40 cultivars of wheat, which has been popularized above 6 67×10 4hm 2 per year in Sichuan during last 50 years. The results are as follows: 40 arbitrary primers (10-mers) were used for the PCR amplification of random genomic DNA fragments. Some 58 2% (amount to 32) of the primers could amplify polymorphic bands. Out of 185 amplified bands, 93 (about 50%) are polymorphic. Every primer could amplify 1 to 11 polymorphic bands, with an average of 2 9 bands. The mean GD based on RAPD markers among the 40 cultivars is 0 221, with a range from 0 019 to 0 475. Cluster analysis shows that the studied cultivars could be classified into 5 clusters at the level of GD 0 230. Some arbitrary primers could amplify certain cultivars specially. The 9 1BL/1RS wheat rye translocations has special RAPD banding pattern by the primer OPN14. Based on these results,it is concluded that RAPD markers could be used as the fingerprint to identify the wheat cultivars.
出处 《四川农业大学学报》 CSCD 1999年第4期354-361, ,共8页 Journal of Sichuan Agricultural University
基金 四川省科委 教委资助
关键词 小麦 品种 RAPD标记 遗传距离 聚类分析 WHEAT (T.AESTIVUM L.) CULTIVARS RAPD GENETIC DISTANCE CLUSTER ANALYSIS
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