提取大白菜基因组DNA的几种方法比较

Comparison of genomic DNA extraction methods of Chinese cabbage ( Brassica campestris ssp. pekinensis )

对用 SDS法、尿素法、改良 SDS法、氯化苄和 CTAB法提取的大白菜基因组DNA进行了比较。结果表明 ,改良 SDS法提取的 DNA具有典型的天然 DNA分子的标准紫外吸收光谱特点 ,其 A2 6 0 /A2 80 为 1 .7～ 1 .9,A2 6 0 /A2 30 为 1 .8～ 2 .0 ,叶片的 DNA产量为 40 0μg/g。用该法提取的大白菜 DNA适于进行 RAPD分析。

The DNA samples of Chinese cabbage male sterile line “CMS3411 7” and maintainer line “3411 7” were prepared by SDS ,Urea,improved SDS,Benzyl chloride and CTAB methods,respectively.The results showed that DNA samples obtained by the improved SDS method possessed the standard ultraviolet absorbance spectrum of pure natural DNA,whose A 260 /A 280 was 1.70-1.90 and A 260 /A 230 was 1.8-2.0.The yield of DNA obtained by the improved SDS method was 400 μg/g young leaves,and the DNA samples prepared by this procedure were suitable for RAPD analysis.