塞来昔布对人鼻咽癌细胞凋亡的促进作用

Promote effects of cyclooxygenase-2 inhibitor celecoxib on the apoptosis of nasopharyngeal carcinoma cells

目的研究塞来昔布对人鼻咽癌CNE细胞凋亡的促进作用。方法以人鼻咽癌细胞株CNE为研究对象,设实验组(加入塞来昔布20mmol/L)及对照组,培养48h后,制备成超薄切片透射电镜观察塞来昔布对细胞凋亡的影响。取不同浓度塞来昔布(0×10-5mol/L、1.0×10-5mol/L、2.0×10-5mol/L、4.0×10-5mol/L)处理细胞,用流式细胞仪检测细胞周期分布及凋亡。TUNEL(DNA末端原位标记染色法)荧光染色法检测CNE细胞凋亡,计算凋亡率%=凋亡阳性细胞数/100个CNE细胞。结果给予塞来昔布48 h后,透射电镜下CNE细胞染色质固缩边集,细胞器肿胀,胞浆内可见凋亡小体形成。流式细胞学结果显示塞来昔布对CNE细胞周期分布的影响S期细胞减少,G1期细胞增加,经浓度分别10mmol/L、20mmol/L、40mmol/L塞来昔布处理48h,随浓度增大,凋亡率增加。TUNEL结果显示:实验组凋亡细胞明显增多,CNE细胞凋亡百分比(4.3±2.21)%,明显高于对照组(0.9±0.99)%,差异有统计学意义(P<0.01)。结论本实验将塞来昔布作用于人鼻咽癌细胞株CNE细胞后,运用电镜观察到凋亡细胞增加。不同浓度的塞来昔布作用于人鼻咽癌细胞株CNE细胞后,流式细胞术、TUNEL荧光染色均证明环氧合酶-2抑制剂塞来昔布对凋亡有促进作用,塞来昔布(特异性环氧合酶-2抑制剂)已被认为肿瘤治疗新靶标,可为临床的肿瘤治疗提供新的方法。

Objective To explore the effection of celecoxib on the apoptosis of nasopharyngeal carcinoma. Methods In vivo cultured CNE cells of nasopharyngeal carcinoma were treated with different concentration celecoxib, a selective COX-2 inhibi- tor. TEM （transmission electron microscope）, flow cytometryassay （FCM） and TUNEL were used to measure the apoptosis. Re- suits There were more apoptosis body in the celecoxib group than control group in TEM. The S phase ceils decreased and G phase ceils increased in FCM of the celecoxib group, Celecoxib may inhibited the G cells to S phase transition. There was a does-dependent induction of cell apoptosis by celecoxib. Apoptosis rate with celecoxib is much higher than that without celecoxib in CNE cells by TUNEL （P 〈 0. 01 ）. Conclusion Celecoxib induced apoptosis tested by the measurement of TEM , flow cytom- etryassay and TUNEL. It may be a new interfering target of celecoxib on the treatment of NPC.