Sox2、Klf4、Oct4、c-Myc基因编程脐带基质间充质细胞

Umbilical cord matrix cells were reprogrammed bySox2、Klf4、Oct4、c-Myc Genes

目的探讨利用Sox2、Klf4、Oct4、c-Myc基因将脐带基质间充质细胞(UMC)编程为多潜能干细胞(iPS)。方法原代分离培养UMC细胞,包装生产逆转录病毒感染细胞,将感染后细胞接种到饲养层细胞培养,镜下观察细胞形态学变化。对编程后细胞进行碱性磷酸酶(AP)染色、检测细胞内源多能基因表达量和体内分化畸胎瘤实验。结果原代获得UMC细胞,被编程细胞形态类似于胚胎干细胞,AP染色阳性,内源多能基因Oct4、Sox2、Nanog、Rex1表达量增高,体内分化为畸胎瘤。结论利用Sox2、Klf4、Oct4、c-Myc基因可将UMC细胞编程为iPS细胞。

Objective Umbilical cord matrix cells （UMC） were reprogrammed to induced pluripotent stem cells （iPS） by Sox2 ,Klf4,Oct4,c-Myc genes. Methods UMC were isolated and cultured in vitro. UMC were infected by packaging and pro- ducing retroviral supernatant with Sox2 ,Klf4 ,Oct4 ~c-Myc genes. They were cultured in the feeder layer cell after the ceils infected by retrovirus. The cell morphology were observed through microscope during reprogramming the cells. The cells were evaluated by Alkaline phosphatase staining^endogenous gene expression,teratomas of differentiation experiment in vivo. Results It was suc- cessful to obtain the UMC cells. The cell morphology was similar to embryonic stem cells. AP staining was positive, endogenous pluripotency genes（ Oct4, Sox2, Nanog, Rexl ） expression increased, the cells can be differentiated into teratomas in vivo. Con- clusion Generation of human induced pluripotent stem cells from umbilical cord matrix cells.