基于ILs玉米抗旱相关位点分析

Analysis of Drought-resistant Loci by ILs in Maize

选用抗旱性优良的玉米自交系郑独青为供体,采用高代回交方法构建以自交系昌7-2为主要遗传背景的导入系群体,通过SSR标记检测群体中供体等位基因的导入频率,利用卡方检验对等位基因导入频率的偏离进行检测,同时结合表型-基因型方差分析的方法,对抗旱群体进行抗旱性相关位点分析。结果表明,卡方检验发现显著位点33个,方差分析检测到24个,其中15个位点被两种方法同时检测到。

ILs mapping populations deriving from Chang7-2 and Zhengduqing were constructed under drought stress. The population were used in the present study to detect genomic regions for drought tolerance at vegetative stage by the use of SSR markers and statistical methods such as X 2 test and ANOVA, thirty-three belonged to allelic frequency-deviation loci detected by chi-square test and 24 were detected by analysis of variances（ANOVA） with data from progeny test in replicated experiments. Fifteen loci were detected both by chi-square test and ANOVA.