玉米转脂蛋白基因ZmLTP3的克隆及表达特性分析

Cloning and Characterization of a Lipid Transfer Protein Gene, ZmLTP3, from Maize

转脂蛋白(Lipid transfer proteins,LTPs)是广泛存在于植物中的一类小分子蛋白,在植物生长发育及逆境应答中起着十分重要的作用。通过RT-PCR方法从玉米中克隆1个与拟南芥LTP3基因同源的全长cDNA,命名为ZmLTP3。推测ZmLTP3蛋白含有123个氨基酸,分子量为12.5 kDa,等电点为9.38。半定量RT-PCR分析表明,ZmLTP3可以被甘露醇、高盐和水杨酸(SA)诱导。在不同组织中ZmLTP3的表达模式不同,在子房中的表达量最高。结果表明,ZmLTP3可能在植物逆境反应及生殖生育过程中起多重作用。

Plant lipid transfer proteins（LTPs） are encoded by muhigene families and play important roles in plant development and stress responses. One full-length cDNA encoding an A rabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. The predicted ZmLTP3 protein has 123 amino acids with an estimated molecular mass of 12.5 kD and an isoelectric point of 9.38. RT-PCR analysis indicated that the ZmLTP3 expression is induced by mannitol, high salt and salicylic acid（SA）. Furthermore, in different tissues the ZmLTP3 displayed different expression patterns, and was highest in ovaries, indicated that ZmLTP3 may play multiple roles stresses resistance and development.