发现于炎症区域分子1在实验性大鼠肺纤维化组织中的表达及意义

Expression and significance of found in inflammatory zone 1 in pulmonary fibrosis of experimental rats

目的研究发现于炎症区域分子1(FIZZ1)在大鼠肺纤维化组织中的表达及意义。方法 10只SpragueDawley大鼠随机分为模型组和对照组,每组5只。大鼠采用气管内注入博来霉素(5 mg·kg-1)的方法复制大鼠肺纤维化模型(模型组);采用气管内注入生理盐水(1.25 mL·kg-1)的方法制造阴性对照(对照组)。第28天处死大鼠,肺组织病理切片行苏木精-伊红染色,Ashcroft评分评定大鼠肺组织纤维化的严重程度;免疫组织化学方法检测大鼠FIZZ1和转化生长因子-β1(TGF-β1)的表达。结果模型组大鼠肺纤维化程度较对照组严重,Ashcroft评分比较差异有统计学意义(P<0.01);模型组大鼠FIZZ1和TGF-β1表达水平较对照组升高,差异有统计学意义(P<0.01)。结论 FIZZ1在肺纤维化发病机制中起重要作用,可能与TGF-β1的高表达有关。

Objective To study the expression and significance of found in inflammatory zone 1 （FIZZ1）in pulmonary fibrosis of experimental rats. Methods Ten Sprague-Dawley rats were randomly divided into control group （ n = 5 ） and model group（ n = 5 ）. Pulmonary fibrosis model was induced by using the method of intratracheal instillation of bleomycin （5 mg · kg-1 ）. Control group was treated by intratracheal instillation of saline （ 1.25 mL · kg- 1 ） to obtain the negative control. On the twenty-eighth day, all rats were sacrificed and the lung tissues were harvested. The lung tissue was performed with hematoxylineosin staining. The severity of pulmonary fibrosis in rats was assessed by Ashcroft score. Immunohistochemical staining was used to detect the expression of FIZZ1 and transforming growth factor-β1 （TGF-β1）. Results Compared with the rats of control group, the severity of pulmonary fibrosis was increased in rats of model group, and there was significant difference in Ashcroft score（P 〈 0. 01 ）. The expression levels of FIZZ1 and TGF-β1 in model group were higher than those in control group （ P 〈 0.01 ）. Conclusion FIZZ1 may play an important role in the development of pulmonary fibrosis, impossibly relates with TGF-β1 overexpression.