摘要
在正确提取DNA后,探讨了在成本最低、效果最好的基础上,得出了DNA最佳增殖反应液的组成(10Xbuffer1.50、dntps 0.30、MgCl2 0.60、灭菌水 10.57、Primer1.00、Tag0.03、DNA1.00),其次用 60个引物对杨柳科属和杨属的 13个品种进行了人工分析,发现多态标记 32个,用其中的任意 13个标记就能区别 13品种。
The best reaction composition have been studied in this paper. It is that 10xbuffer 1. 50μl,dNTP 0. 30μl,MgCl2 1. 60μl,h20 10. 57μl,primer 1. 00 μl,TaqDNA 0. 03μl.Template DNA 1. 00μl. The results show htat: 32 polymorphic markers have been found when 60 primers were used to identify the 13 varieties of Populus,and 13 arbitrary markers can identify them completely.
出处
《湖北林业科技》
2000年第C00期8-13,共6页
Hubei Forestry Science and Technology