摘要
在正确提取ＤＮＡ后，探讨了在成本最低、效果最好的基础上，得出了ＤＮＡ最佳增殖反应液的组成（１０Ｘｂｕｆｆｅｒ１．５０、ｄｎｔｐｓ ０．３０、ＭｇＣｌ２ ０．６０、灭菌水 １０．５７、Ｐｒｉｍｅｒ１．００、Ｔａｇ０．０３、ＤＮＡ１．００），其次用 ６０个引物对杨柳科属和杨属的 １３个品种进行了人工分析，发现多态标记 ３２个，用其中的任意 １３个标记就能区别 １３品种。
The best reaction composition have been studied in this paper. It is that 10xbuffer 1. 50μl,dNTP 0. 30μl,MgCl2 1. 60μl,h20 10. 57μl,primer 1. 00 μl,TaqDNA 0. 03μl.Template DNA 1. 00μl. The results show htat: 32 polymorphic markers have been found when 60 primers were used to identify the 13 varieties of Populus,and 13 arbitrary markers can identify them completely.
出处
《湖北林业科技》
2000年第C00期8-13,共6页
Hubei Forestry Science and Technology
