eIF4E在食管鳞状细胞癌中表达及其与预后关系的研究

eIF4E expression and its correlationwith prognosisin esophageal squamouscell carcinoma

目的:研究真核细胞翻译起始因子4E(eukaryotic translation initiation factor 4E,eIF4E)在食管癌的表达及其与预后的关系。方法:采用RT-PCR及免疫组化SP法检测食管癌组织及正常食管黏膜组织中eIF4E的表达,分析其表达与食管癌临床病理特征及预后的关系。结果:eIF4E mRNA在食管癌及癌旁组织中相对表达量分别为0.561±0.079,0.442±0.061(P<0.05);不同分化的食管鳞癌eIF4E表达量不同(P=0.012),多重比较显示低分化鳞癌的表达量高于高分化鳞癌的表达(P<0.05);不同浸润深度的eIF4E mRNA的表达量不同(P=0.003),多重比较显示在深肌层和纤维膜的表达量高于黏膜层的表达量(P<0.05)。eIF4E蛋白在食管癌和正常食管黏膜中的阳性表达率分别为75.00%、30.80%(P=0.001);与病理学分级呈正相关(r s=0.228,P<0.05);不同浸润深度的eIF4E蛋白表达量不同(P<0.05)。而两者均与食管癌的患者年龄、性别、淋巴结转移和临床分期等临床病理学特征无关(P>0.05)。eIF4E蛋白高表达组及低表达组5年累积生存率分别为31.8%、53.3%(P<0.05)。Cox模型分析得出eIF4E蛋白不能作为判断食管鳞癌预后的独立指标(P=0.211)。结论:eIF4E表达与食管鳞状细胞癌的发生密切相关,其可能参与了肿瘤的浸润和发展,并可以和其他指标共同判断预后。

Objective：To investigate the expression of eukaryotic translation initiation factor 4E （eIF4E） in the occurrence and development of esophageal squamous cell carcinoma （ESCC）and the correlation between eIF4E and prognosis.Methods：eIF4E mRNA and protein expression in esophageal carcinoma and normal esophageal mucosa were detected by reverse transcriptase polymerase chain reaction （RT-PCR） and immunohistochemical （IHC） SP method.Results：The level of eIF4E mRNA expression in ESCC was 0.561±0.079,significantly higher than that in adjacent esophageal mucosa 0.442±0.061 （P〈0.05）,the levels of eIF4E mRNA relative expression were influenced by pathological grading of the tumor （P=0.012）,and multiple comparisons showed that the expression was higher in low-differentiated group than in well-differentiated group （P〈0.05）.eIF4E mRNA expression in different invasion grade were different （P=0.003）,and multiple comparisons showed that higher in fiber membrane and deep muscle than in mucous layer （P〈0.05）.The immunohistochemistry results：the positive expression rate of eIF4E was 75.00%,significantly higher than in esophageal mucosa 30.80% （P=0.001）.There was a positive correlation with pathological grading of the tumor （rs=0.228,P〈0.05）.There was correlation with invasive depth （P〈0.05）,but not with gender,age,lymph node metastasis and different clinical stages （P〉0.05）.5-year survival rate of patients with high expression of eIF4E was 31.8%,significantly lower than the 53.3% with low expression group （P〈0.05）.The Cox model analysis showed that the expression of eIF4E influenced the prognosis of ESCC,so it can be used as supplementary indicator of prognosis.Conclusion：eIF4E expression had the role in the occurrence of esophageal squamous cell carcinoma,and may be involved in tumor invasion and development.The enhanced expression of eIF4E as a reference index of ESCC indicated poor prognosis.