PTEN、p53和EGFR在乳腺癌中的表达与临床意义

Expression and clinical significance of PTEN,p53 and EGFR in breast carcinoma

目的:检测PTEN、p53和EGFR在乳腺癌中的表达,分析它们与乳腺癌临床病理特征的关系及三者的相关性,并探讨其与乳腺癌预后的关系。方法:采用免疫组织化学SP法,检测PTEN、p53和EGFR在68例乳腺浸润性癌和10例乳腺纤维腺瘤组织标本中的表达。结果:PTEN、p53和EGFR在乳腺癌组织中的阳性率为45.6%、51.5%和58.8%,PTEN在乳腺癌组织中的表达明显低于纤维腺瘤组织,p53和EGFR在乳腺癌组织中的表达明显高于纤维腺瘤组织,差异有统计学意义(P<0.05)。PTEN、p53和EGFR蛋白在乳腺癌中表达与组织学分级、淋巴结转移情况、雌激素受体(ER)状态均有关(P<0.05)。PTEN和p53阳性率在死亡组与生存组的差异有统计学意义(P<0.05);EGFR阳性率在死亡组中高于生存组,但差异无统计学意义(P>0.05)。结论:PTEN表达的缺失和p53、EGFR的过度表达共同参与了乳腺癌的转移和进展,它们与肿瘤的侵袭性和预后有关。三者联合检测有助于乳腺癌的早期病理诊断、恶性程度的判断及预后的评估。

Objective：To evaluate the significance of PTEN,p53 and EGFR in the invasive breast cancer and the relationship between these markers and clinieopathologic parameter as well as clinical outcome. Methods： SP immunohistochemistry was used in detecting the expression of PTEN,p53 and EGFR in 68 eases of breast carcinoma and 10 eases of breast fibroadenoma. Results：The positive expression rate of PTEN, p53 and EGFR protein in breast carcinoma were 45.6% ,51.5% and 58.8% respectively,which were significantly different than that in breast fibroadenoma （P 〈 0.05）. The expression of PTEN, p53 and EGFR in breast cancer was correlated with histological grade,lymph node metastasis and the status of ER（P 〈 0.05）. The positive expression rate of PTEN in the death group was lower than that in the s urviwl group,which had statistically significant difference （P 〈 0.05 ） ; the positive expression rate of p53 （P 〈 0.05） and EGFR（P 〉 0.05） in the death group is significantly higher than that in the survival group. Conclusion：The deletion of PTEN as well as up - regulated expression of p53 and EGFR are involved in progression and invasion of breast carcinoma and indicate poor prognosis. The combined detection of these three markers could indicate early pathological diagnosis and prognosis of breast carcinoma.