藏药柳茶叶乙醇水提取物中化学成分的鉴定

Determination of the Chemical Constituents in Aqueous Alcohol Extract of Sibiraea Angustata(Rehd.) Leaf Hand-Mazz.

采用多种色谱分离技术对藏药柳茶叶80%乙醇提取物进行分离纯化,得到了9个化合物。根据其理化性质与NMR谱数据对化合物进行了结构鉴定,分别为金丝桃苷(hyperoside,Ⅰ)、槲皮素(quercetin,Ⅱ)、芸香苷(rutin,Ⅲ)、槲皮素3-O-β-D-吡喃葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,Ⅳ)、儿茶素(catechin,Ⅴ)、香草酸(vanillic acid,Ⅵ)、熊果苷(arbutin,Ⅶ)、异阿魏酸(isoferulic acid,Ⅷ)和胡萝卜苷(daucosterol,Ⅸ)。化合物中除Ⅳ、Ⅷ和Ⅸ外,其余6个化合物为首次从该属植物中分离鉴别。

To determine the chemical constituents from Sibiraea angustata（Rehd.） Hand-Mazz, its 80% ethanol extract was isolated and purified by chromatograph over silica gel, Sephadex LH-20 and HPLC, etc. Nine compounds were isolated and identified as hyperoside（Ⅰ）, quercetin（Ⅱ）, rutin（Ⅲ）, quercetin-3-O-β-D-glucopyranoside（Ⅳ）, catechin（Ⅴ）, vanillic acid（Ⅵ）, arbutin（Ⅶ）, isoferulic acid（Ⅷ）, daucosterol（Ⅸ）. Their chemical structures were determined by chemical methods, NMR spectral data. Compounds Ⅰ-Ⅲ and Ⅴ-Ⅶ were isolated from the leaves of Sibiraea angustata（Rehd.） Hand-Mazz. for the first time.