武汉市部分菜场肉类食品中产志贺毒素大肠杆菌污染状况分析

Analysis on contamination status of Shiga toxin-producing Escherichia coli in retail meats from some markets in Wuhan

目的对武汉市部分菜场肉类食品中产志贺毒素大肠杆菌(STEC)污染状况进行分析。方法 2011年7月至2012年10月期间,从武汉市汉口30个菜场和超市共采集样品196份,其中猪肉102份,牛肉60份,禽类34份。通过选择性增菌,提取DNA,PCR扩增stx1、stx2、rfb O157、wzy O157基因,分析食品中产志贺毒素大肠杆菌的污染状况。结果猪肉、牛肉、禽类食品中非O157型STEC的阳性检出率分别为18.6%、48.4%和2.9%;O157型STEC的阳性检出率分别为13.6%(猪肉)、6.7%(牛肉)和2.9%(禽类)。菜场样品STEC检出率(35.8%)略高于超市样品(32.4%)。结论武汉市肉类食品中STEC检出率较高,应定期跟踪监测,了解菌株流行和毒力变化状况。

Objective To investigate the contamination of Shiga toxin-producing Escherichia coli （STEC） in retail meats in Wuhan city. Methods A total of 196 samples were collected from 30 open markets and supermarkets during a two-year sampling period （from 2011 July to 2012 October）. Specimens comprised 102 pork, 60 beef and 34 poultry samples. All samples were performed selective incubation, then screened by PCR amplification for the presence of stxl, stx2, rfbO157 and wzyo157 genes. Results Shiga toxin-producing Escherichia coli （STEC） were frequently detected in beef, pork and pouhry products. 18.6% raw meats, 48.4% beef and 2. 9% poultry samples had been tested positive for the presence of non- O157 STEC. The detection rate of O157 type STEC was 13.6% in pork, 6.7% in beef and 2.9% in poultry, respectively. The detection rate of STEC in open markets （35.8%） was a little higher than that in supermarkets （32.4%）. Conclusion The STEC detection rate is a little high in retail meats in Wuhan, and it should be concerned.