基于硼酸基功能化的磁性表面印迹聚合物制备及其对目标糖蛋白的识别

Preparation of boronic acid functionalized magnetic surface molecularly imprinted polymer and its recognition of the target glycoprotein

以硼酸基功能化的磁性Fe3O4纳米粒子(Fe3O4NPs)为载体,卵清蛋白(OB,一种糖蛋白)作模板分子,多巴胺(DA)为单体,采用表面印迹的方法,制备了一种磁性表面分子印迹聚合物纳米粒子。用平衡吸附实验研究了其吸附性能和识别选择性。结果表明,该分子印迹聚合物对目标糖蛋白(OB)具有较高的选择性和吸附量,并且具有良好的磁性,有利于进行快捷的磁性分离,这将为复杂生物样品中目标糖蛋白的专一性识别提供一种新的途径。

Using boronic acid-functionalized magnetic Fe3O4 nanoparticles （NPs） as supports, ovalbumin （OB） as the model glycoprotein, dopamine （DA） as the monomer, the magnetic surface molecularly imprinted polymers （MIPs） NPs were prepared by surface imprinting. The recognition and selectivity of the MIPs were studied by equilibrium adsorption experiments. The results obtained showed that the imprinted polymers exhibited high adsorption capacity and good recognition capability for OB, and the high saturation magnetization of the magnetic MIPs was very favorable for rapid magnetic separation. This will provide a new way for specific recognition of the target glycoprotein in the complex biological samples.