摘要
建立了功能食品中8种有机酸同时测定的方法。样品中的有机酸(乙酸、丙酸、丁酸、草酸、乳酸、丙二酸、丁二酸、柠檬酸)经体积分数15%的硫酸甲醇提取酯化,三氯甲烷萃取,最后用气相色谱法测定。色谱条件:SPB-5毛细管柱(30m×0.53mm×0.5μm)为分离柱,火焰离子化检测器检测,进样口温度为250oE,检测器温度为300℃,程序升温。载气(N2)流速为0.8mL/min,分流比为10:1,富马酸二甲酯内标法定量。8种有机酸衍生物达到基线分离,检测限范围为0.01~0.04mg/L,精密度(RSD)范围为2.4%~5.5%,平均回收率范围为79.5%~106%。方法适合于功能食品中8种有机酸的快速测定。
A method was established for simultaneous determination of 8 organic acids including acetic acid, propionic acid, butyric acid, oxalic acid, lactic acid, malonic acid, succinic acid and citric acid in functional foods by gas chromatography with pre-column derivatization and liquid phase microextraction. The organic acids in samples were extracted and esterified with 15% sulfuric acid in methanol, and microextracted with chloroform, and finally determined with gas chromatography. Chromatographic separation was performed on a SPB-5 capillary column(30 ×0.53mm×0.5μm)with FID as the detector. The injection temperature was 250 ℃, and the detector temperature was 300 ~C ,with temperature programming. N2was used as carrier gas with a flow rate of 0. 8 mL/min and split ratio was 10: 1. Dimethyl fumarate internal method was used for quantification. The baseline resolution was achieved for all the derivatives of eight organic acids. The detection limits were 0. 01 ~0. 04 mg/L, the precisions(RSDs) were 2. 4% - 5.5% , and the average recoveries were 79.5% ~ 106%. The method is accurate and rapid, and can be used for simulations determination of 8 organic acids in functional foods.
出处
《分析试验室》
CAS
CSCD
北大核心
2014年第2期175-179,共5页
Chinese Journal of Analysis Laboratory
基金
科技部"十二五"支撑计划课题(2012BAD33B02)资助
关键词
有机酸
气相色谱法
衍生化
功能食品
Organic acid
Gas chromatography
Derivatization
Functional food